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Molecules 2015, 20(5), 7957-7973; doi:10.3390/molecules20057957

Effective Immobilization of Agrobacterium sp. IFO 13140 Cells in Loofa Sponge for Curdlan Biosynthesis

1
Postgraduate Program in Food Science, State University of Maringá (UEM), Av. Colombo, 5790, Maringá-PR 87020-900, Brazil
2
Pharmacy Department, State University of Maringá (UEM), Av. Colombo, 5790, Maringá-PR 87020-900, Brazil
3
Food Engineering Department, Federal Technological University of Paraná (UTFPR), Via Rosalina Maria dos Santos, 1233, Campo Mourão-PR 87301-899, Brazil
*
Author to whom correspondence should be addressed.
Academic Editor: Maurizio Battino
Received: 28 January 2015 / Revised: 20 April 2015 / Accepted: 28 April 2015 / Published: 4 May 2015
(This article belongs to the Section Natural Products)
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Abstract

Curdlan production by Agrobacterium sp. IFO13140 immobilized on loofa sponge, alginate and loofa sponge with alginate was investigated. There was no statistically-significant difference in curdlan production when the microorganism was immobilized in different matrices. The loofa sponge was chosen because of its practical application and economy and because it provides a high stability through its continued use. The best conditions for immobilization on loofa sponge were 50 mg of cell, 200 rpm and 72 h of incubation, which provided a curdlan production 1.50-times higher than that obtained by free cells. The higher volumetric productivity was achieved by immobilized cells (0.09 g/L/h) at 150 rpm. The operating stability was evaluated, and until the fourth cycle, immobilized cells retained 87.40% of the production of the first cycle. The immobilized cells remained active after 300 days of storage at 4 °C. The results of this study demonstrate success in immobilizing cells for curdlan biosynthesis, making the process potentially suitable for industrial scale-up. Additional studies may show a possible contribution to the reduction of operating costs. View Full-Text
Keywords: curdlan; Agrobacterium sp.; immobilization; mixed-level design; loofa sponge curdlan; Agrobacterium sp.; immobilization; mixed-level design; loofa sponge
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

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MDPI and ACS Style

Martinez, C.O.; Ruiz, S.P.; Nogueira, M.T.; Bona, E.; Portilho, M.; Matioli, G. Effective Immobilization of Agrobacterium sp. IFO 13140 Cells in Loofa Sponge for Curdlan Biosynthesis. Molecules 2015, 20, 7957-7973.

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