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Molecules 2015, 20(4), 6093-6112; doi:10.3390/molecules20046093

Various Extraction Methods for Obtaining Stilbenes from Grape Cane of Vitis vinifera L.

1
Department of Post-Harvest Technology of Horticultural Products, Faculty of Horticulture, Mendel University in Brno, Valtická 337, Lednice 69144, Czech Republic
2
Global Change Research Centre, Academy of Sciences of the Czech Republic, v. v. i., Branišovská 31, České Budějovice 37005, Czech Republic
3
Institute of Chemical Process Fundamentals, Academy of Sciences of the Czech Republic, v.v.i., Rozvojová 2/135, 16502 Prague 6, Czech Republic
*
Author to whom correspondence should be addressed.
Academic Editor: Derek J. McPhee
Received: 16 February 2015 / Revised: 30 March 2015 / Accepted: 2 April 2015 / Published: 8 April 2015
(This article belongs to the Section Natural Products)
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Abstract

Grape cane, leaves and grape marc are waste products from viticulture, which can be used to obtain secondary stilbene derivatives with high antioxidant value. The presented work compares several extraction methods: maceration at laboratory temperature, extraction at elevated temperature, fluidized-bed extraction, Soxhlet extraction, microwave-assisted extraction, and accelerated solvent extraction. To obtain trans-resveratrol, trans-ε-viniferin and r2-viniferin from grape cane of the V. vinifera variety Cabernet Moravia, various conditions were studied: different solvents, using powdered versus cut cane material, different extraction times, and one-step or multiple extractions. The largest concentrations found were 6030 ± 680 µg/g dry weight (d.w.) for trans-resveratrol, 2260 ± 90 µg/g d.w. for trans-ε-viniferin, and 510 ± 40 µg/g d.w. for r2-viniferin. The highest amounts of stilbenes (8500 ± 1100 µg/g d.w.) were obtained using accelerated solvent extraction in methanol. View Full-Text
Keywords: Vitis vinifera L.; grape cane; stilbenes; accelerated solvent extraction (ASE); microwave-assisted extraction (MAE); LC-MS Vitis vinifera L.; grape cane; stilbenes; accelerated solvent extraction (ASE); microwave-assisted extraction (MAE); LC-MS
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

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MDPI and ACS Style

Soural, I.; Vrchotová, N.; Tříska, J.; Balík, J.; Horník, Š.; Cuřínová, P.; Sýkora, J. Various Extraction Methods for Obtaining Stilbenes from Grape Cane of Vitis vinifera L.. Molecules 2015, 20, 6093-6112.

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