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Molecules 2015, 20(4), 6060-6067; doi:10.3390/molecules20046060

Development and Characterization of Polymorphic Genic-SSR Markers in Larix kaempferi

1
State Key Laboratory of Tree Genetics and Breeding, Chinese Academy of Forestry, Xiangshan Rd., Beijing 100091, China
2
Research Institute of Forestry, Chinese Academy of Forestry, Xiangshan Rd., Beijing 100091, China
*
Author to whom correspondence should be addressed.
Academic Editor: Derek J. McPhee
Received: 14 February 2015 / Revised: 24 March 2015 / Accepted: 26 March 2015 / Published: 8 April 2015
(This article belongs to the Section Molecular Diversity)
View Full-Text   |   Download PDF [675 KB, uploaded 8 April 2015]   |  

Abstract

New simple sequence repeat (SSR) markers were developed in the Japanese larch (Larix kaempferi) using unigene sequences for further genetic diversity studies and the genetic improvement of breeding programs. One thousand two handred and thirty five (1235) primer pairs were tested and 165 successfully identified in L. kaempferi. Out of the amplified candidate markers, 145 (90.6%) exhibited polymorphism among 24 individuals of L. kaempferi, with the number of alleles per locus (Na), observed heterozygosity (Ho), expected heterozygosity (He) and polymorphic information content (PIC) averaging at 4.510, 0.487, 0.518 and 0.459, respectively. Cross-species amplification of randomly selection of 30 genic-SSRs among the 145 polymorphic ones showed that 80.0% of the SSRs could be amplified in Larix olgensis, 86.7% could be amplified in Larix principi-rupprechtii and 83.0% could be amplified in Larix gmelinii. High rates of cross-species amplification were observed. The genic-SSRs developed herein would be a valuable resource for genetic analysis of Larix kaempferi and related species, and also have the potential to facilitate the genetic improvement and breeding of larch. View Full-Text
Keywords: Larix kaempferi; unigene sequences; simple sequence repeat (SSR); polymorphism; cross-species amplification Larix kaempferi; unigene sequences; simple sequence repeat (SSR); polymorphism; cross-species amplification
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

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MDPI and ACS Style

Chen, X.-B.; Xie, Y.-H.; Sun, X.-M. Development and Characterization of Polymorphic Genic-SSR Markers in Larix kaempferi. Molecules 2015, 20, 6060-6067.

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