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Molecules 2015, 20(2), 3238-3254; doi:10.3390/molecules20023238

Toxicity of Amorphigenin from the Seeds of Amorpha fruticosa against the Larvae of Culex pipiens pallens (Diptera: Culicidae)

1
Department of Pesticide Science, College of Plant Protection, Shenyang Agricultural University, Shenyang 110866, China
2
Biopesticide Engineering Research Center of Liaoning Province, Shenyang 110866, China
*
Authors to whom correspondence should be addressed.
Academic Editor: Derek J. McPhee
Received: 16 December 2014 / Revised: 5 February 2015 / Accepted: 10 February 2015 / Published: 16 February 2015
(This article belongs to the Section Natural Products)
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Abstract

The larvicidal activity of the crude petroleum ether, ethyl acetate, acetone, chloroform and ethanol extracts of Amorpha fruticosa seeds was individually assayed for toxicity against the early fourth-instar larva of the mosquito, Culex pipiens pallens after 24 h exposure. Of the tested extracts, the ethanol one exhibited the highest larvicidal activity (LC50 = 22.69 mg/L). Amorphigenin (8'-hydroxyrotenone), a rotenoid compound which exhibits a strong larvicidal activity with LC50 and LC90 values of 4.29 and 11.27 mg/L, respectively, was isolated from the ethanol extract by column chromatograpy. Its structure was elucidated by 1H-NMR, UV and IR spectral data. Furthermore, investigation of amorphigenin’s effects on mitochondrial complex I activity and protein synthesis in C. pipiens pallens larvae reveals that amorphigenin decreases mitochondrial complex I activities to 65.73% at 10.45 μmol/L, compared to the control, when NADH were used as the substrate. Meanwhile, amorphigenin at 10.45 μmol/L also caused a 1.98-fold decrease in protein content, compared to the control larvae treated with acetone only. View Full-Text
Keywords: Amorpha fruticosa; larvicidal activity; Culex pipiens pallens; amorphigenin; mitochondrial complex I; protein synthesis Amorpha fruticosa; larvicidal activity; Culex pipiens pallens; amorphigenin; mitochondrial complex I; protein synthesis
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

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MDPI and ACS Style

Liang, Y.; Li, X.; Gu, Z.; Qin, P.; Ji, M. Toxicity of Amorphigenin from the Seeds of Amorpha fruticosa against the Larvae of Culex pipiens pallens (Diptera: Culicidae). Molecules 2015, 20, 3238-3254.

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