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Molecules 2015, 20(12), 22621-22634; doi:10.3390/molecules201219873

Analysis of Indole Alkaloids from Rhazya stricta Hairy Roots by Ultra-Performance Liquid Chromatography-Mass Spectrometry

1
VTT Technical Research Centre of Finland Ltd., P.O. Box 1000, FI-02044 VTT, Finland
2
Division of Pharmaceutical Biosciences, Faculty of Pharmacy, P.O. Box 56, FI-00014 University of Helsinki, Finland
*
Author to whom correspondence should be addressed.
Academic Editor: Thomas J. Schmidt
Received: 28 October 2015 / Revised: 10 December 2015 / Accepted: 11 December 2015 / Published: 17 December 2015
(This article belongs to the Section Natural Products)
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Abstract

Rhazya stricta Decne. (Apocynaceae) contains a large number of terpenoid indole alkaloids (TIAs). This study focused on the composition of alkaloids obtained from transformed hairy root cultures of R. stricta employing ultra-performance liquid chromatography-mass spectrometry (UPLC-MS). In the UPLC-MS analyses, a total of 20 TIAs were identified from crude extracts. Eburenine and vincanine were the main alkaloids followed by polar glucoalkaloids, strictosidine lactam and strictosidine. Secodine-type alkaloids, tetrahydrosecodinol, tetrahydro- and dihydrosecodine were detected too. The occurrence of tetrahydrosecodinol was confirmed for the first time for R. stricta. Furthermore, two isomers of yohimbine, serpentine and vallesiachotamine were identified. The study shows that a characteristic pattern of biosynthetically related TIAs can be monitored in Rhazya hairy root crude extract by this chromatographic method. View Full-Text
Keywords: Rhazya stricta; terpenoid indole alkaloids; ultra-performance liquid chromatography-mass spectrometry Rhazya stricta; terpenoid indole alkaloids; ultra-performance liquid chromatography-mass spectrometry
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

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MDPI and ACS Style

Akhgari, A.; Laakso, I.; Seppänen-Laakso, T.; Yrjönen, T.; Vuorela, H.; Oksman-Caldentey, K.-M.; Rischer, H. Analysis of Indole Alkaloids from Rhazya stricta Hairy Roots by Ultra-Performance Liquid Chromatography-Mass Spectrometry. Molecules 2015, 20, 22621-22634.

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