Next Article in Journal
Helleborus purpurascens—Amino Acid and Peptide Analysis Linked to the Chemical and Antiproliferative Properties of the Extracted Compounds
Next Article in Special Issue
Phenolic Constituents of Medicinal Plants with Activity against Trypanosoma brucei
Previous Article in Journal
Impact of Natural Juice Consumption on Plasma Antioxidant Status: A Systematic Review and Meta-Analysis
Previous Article in Special Issue
Lewies, A., et al. The Potential Use of Natural and Structural Analogues of Antimicrobial Peptides in the Fight against Neglected Tropical Diseases. Molecules 2015, 20, 15392–15433
Article Menu

Export Article

Open AccessArticle
Molecules 2015, 20(12), 22157-22169; doi:10.3390/molecules201219829

Leishmanicidal Activity of (+)-Phyllanthidine and the Phytochemical Profile of Margaritaria nobilis (Phyllanthaceae)

1
Laboratório de Parasitologia e Biologia Estrutural, Instituto de Ciências Biológicas, Universidade Federal do Pará, Belém, Pará 66075-110, Brazil
2
Instituto Nacional de Biologia Estrutural e Bioimagem, Universidade Federal do Rio de Janeiro, Rio de Janeiro 21941-901, Brazil
3
Programa de Pós-Graduação em Química, Instituto de Ciências Exatas e Naturais, Universidade Federal do Pará, Belém, Pará 66075-110, Brazil
4
Laboratório de Microscopia Eletrônica, Instituto Evandro Chagas, Secretaria de Vigilância em Saúde, Ministério da Saúde, Belém, Pará 66090-000, Brazil
5
Coordenação de Botânica, Museu Paraense Emílio Goeldi, Belém, Pará 66077-901, Brazil
*
Author to whom correspondence should be addressed.
Academic Editor: Thomas J. Schmidt
Received: 7 October 2015 / Revised: 26 November 2015 / Accepted: 27 November 2015 / Published: 11 December 2015
View Full-Text   |   Download PDF [3628 KB, uploaded 11 December 2015]   |  

Abstract

The effects of the Securinega alkaloid (+)-phyllanthidine on Leishmania (L.) amazonensis and the first chemical investigation of Margaritaria nobilis L.f. (Phyllanthaceae) are described. Treating the parasites with this alkaloid caused a dose-dependent reduction in promastigote growth of 67.68% (IC50 82.37 μg/mL or 353 µM) and in amastigote growth of 83.96% (IC50 49.11 μg/mL or 210 µM), together with ultrastructural alterations in the promastigotes. No cytotoxic effect was detected in mammalian cells (CC50 1727.48 µg/mL or CC50 5268 µM). Classical chromatographic techniques and spectral methods led to the isolation and identification of betulinic acid, kaempferol, corilagin, gallic acid and its methyl ester, besides (+)-phyllanthidine from M. nobilis leaves and stems. Margaritaria nobilis is another source of the small group of Securinega alkaloids, together with other Phyllanthaceae (Euphorbiaceae s.l.) species. The low toxicity to macrophages and the effects against promastigotes and amastigotes are suggestive that (+)-phyllanthidine could be a promising antileishmanial agent for treating cutaneous leishmaniasis. View Full-Text
Keywords: Leishmania (L.) amazonensis; leishmanicidal activity; alkaloid (+)-phyllanthidine Leishmania (L.) amazonensis; leishmanicidal activity; alkaloid (+)-phyllanthidine
Figures

Figure 1

This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

Scifeed alert for new publications

Never miss any articles matching your research from any publisher
  • Get alerts for new papers matching your research
  • Find out the new papers from selected authors
  • Updated daily for 49'000+ journals and 6000+ publishers
  • Define your Scifeed now

SciFeed Share & Cite This Article

MDPI and ACS Style

Moraes, L.S.; Donza, M.R.H.; Rodrigues, A.P.D.; Silva, B.J.M.; Brasil, D.S.B.; Zoghbi, M.G.B.; Andrade, E.H.A.; Guilhon, G.M.S.P.; Silva, E.O. Leishmanicidal Activity of (+)-Phyllanthidine and the Phytochemical Profile of Margaritaria nobilis (Phyllanthaceae). Molecules 2015, 20, 22157-22169.

Show more citation formats Show less citations formats

Related Articles

Article Metrics

Article Access Statistics

1

Comments

[Return to top]

Molecules EISSN 1420-3049 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert
Back to Top