Tissue- Specific Expression Analysis of Anthocyanin Biosynthetic Genes in White- and Red-Fleshed Grape Cultivars
Abstract
:1. Introduction
2. Results and Discussion
2.1. Anthocyanin Profiling in the Skin and Flesh of Two Different V. vinifera Cultivars
2.2. The Differential RNA Expression Profile of Anthocyanin Biosynthetic Enzymes in the Skin and Flesh of Two Different V. vinifera Cultivars
DAA a | Total Anthocyanins (mg/kg Fresh Berries) b | %3′5′-OH Anthocyanins c | 3′5′-/3′-OH Anthocyanins d | % Acylated Anthocyanins c | %Methoxylated Anthocyanins c | |||||
---|---|---|---|---|---|---|---|---|---|---|
Yan73 | Skin | Flesh | Skin | Slesh | Skin | Flesh | Skin | Flesh | Skin | Flesh |
61 | 6.95 ± 0.09a e | 6.54 ± 0.35a | 89.17 | 72.33 | 8.23 | 2.61 | 70.80 | 33.43 | 97.36 | 92.82 |
74 | 66.58 ± 1.45a | 92.07 ± 1.44b | 86.66 | 72.36 | 6.50 | 2.62 | 36.82 | 29.83 | 95.61 | 96.40 |
82 | 410.53 ± 19.35b | 479.56 ± 12.54c | 87.18 | 17.63 | 6.80 | 0.21 | 36.82 | 20.07 | 94.37 | 95.22 |
92 | 1470.17 ± 28.19c | 599.77 ± 27.54d | 87.73 | 22.79 | 7.15 | 0.30 | 41.14 | 17.26 | 89.45 | 95.79 |
101 | 1646.05 ± 46.27d | 650.60 ± 3.18d | 87.50 | 21.73 | 7.00 | 0.28 | 41.25 | 19.73 | 90.19 | 95.54 |
Muscat Hamburg | ||||||||||
63 | 5.52 ± 0.24a | ND f | 13.60 | ND | 0.16 | ND | 19.79 | ND | 45.20 | ND |
76 | 80.91 ± 2.08b | ND | 31.31 | ND | 0.46 | ND | 11.51 | ND | 88.60 | ND |
90 | 224.90 ± 6.18c | ND | 32.42 | ND | 0.48 | ND | 11.91 | ND | 91.08 | ND |
105 | 419.63 ± 4.18d | ND | 33.39 | ND | 0.50 | ND | 11.38 | ND | 90.86 | ND |
112 | 371.44 ± 5.55e | ND | 43.61 | ND | 0.77 | ND | 32.14 | ND | 97.14 | ND |
2.3. Correlations between Gene Expression and Anthocyanin Profiles
3. Materials and Methods
3.1. Plant Material
3.2. Extraction of Anthocyanins and HPLC-MS Analysis
3.3. RNA Extraction and cDNA Synthesis
3.4. Real Time Quantitative PCR
3.5. Statistical Analysis
4. Conclusions
Supplementary Materials
Acknowledgments
Author Contributions
Conflicts of Interest
References
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Xie, S.; Song, C.; Wang, X.; Liu, M.; Zhang, Z.; Xi, Z. Tissue- Specific Expression Analysis of Anthocyanin Biosynthetic Genes in White- and Red-Fleshed Grape Cultivars. Molecules 2015, 20, 22767-22780. https://doi.org/10.3390/molecules201219883
Xie S, Song C, Wang X, Liu M, Zhang Z, Xi Z. Tissue- Specific Expression Analysis of Anthocyanin Biosynthetic Genes in White- and Red-Fleshed Grape Cultivars. Molecules. 2015; 20(12):22767-22780. https://doi.org/10.3390/molecules201219883
Chicago/Turabian StyleXie, Sha, Changzheng Song, Xingjie Wang, Meiying Liu, Zhenwen Zhang, and Zhumei Xi. 2015. "Tissue- Specific Expression Analysis of Anthocyanin Biosynthetic Genes in White- and Red-Fleshed Grape Cultivars" Molecules 20, no. 12: 22767-22780. https://doi.org/10.3390/molecules201219883