Fluorescence and Docking Studies of the Interaction between Human Serum Albumin and Pheophytin
AbstractIn the North of Brazil (Pará and Amazonas states) the leaves of the plant Talinum triangulare (popular: cariru) replace spinach as food. From a phytochemical point of view, they are rich in compounds of the group of pheophytins. These substances, related to chlorophyll, have photophysical properties that give them potential application in photodynamic therapy. Human serum albumin (HSA) is one of the main endogenous vehicles for biodistribution of molecules by blood plasma. Association constants and thermodynamic parameters for the interaction of HSA with pheophytin from Talinum triangulare were studied by UV-Vis absorption, fluorescence techniques, and molecular modeling (docking). Fluorescence quenching of the HSA’s internal fluorophore (tryptophan) at temperatures 296 K, 303 K, and 310 K, resulted in values for the association constants of the order of 104 L∙mol−1, indicating a moderate interaction between the compound and the albumin. The negative values of ΔG° indicate a spontaneous process; ΔH° = 15.5 kJ∙mol−1 indicates an endothermic process of association and ΔS° = 0.145 kJ∙mol−1∙K−1 shows that the interaction between HSA and pheophytin occurs mainly by hydrophobic factors. The observed Trp fluorescence quenching is static: there is initial non-fluorescent association, in the ground state, HSA:Pheophytin. Possible solution obtained by a molecular docking study suggests that pheophytin is able to interact with HSA by means of hydrogen bonds with three lysine and one arginine residues, whereas the phytyl group is inserted in a hydrophobic pocket, close to Trp-214. View Full-Text
Scifeed alert for new publicationsNever miss any articles matching your research from any publisher
- Get alerts for new papers matching your research
- Find out the new papers from selected authors
- Updated daily for 49'000+ journals and 6000+ publishers
- Define your Scifeed now
Chaves, O.A.; Amorim, A.P.O.; Castro, L.H.E.; Sant’Anna, C.M.R.; de Oliveira, M.C.C.; Cesarin-Sobrinho, D.; Netto-Ferreira, J.C.; Ferreira, A.B.B. Fluorescence and Docking Studies of the Interaction between Human Serum Albumin and Pheophytin. Molecules 2015, 20, 19526-19539.
Chaves OA, Amorim APO, Castro LHE, Sant’Anna CMR, de Oliveira MCC, Cesarin-Sobrinho D, Netto-Ferreira JC, Ferreira ABB. Fluorescence and Docking Studies of the Interaction between Human Serum Albumin and Pheophytin. Molecules. 2015; 20(10):19526-19539.Chicago/Turabian Style
Chaves, Otávio A.; Amorim, Ana P.O.; Castro, Larissa H.E.; Sant’Anna, Carlos M.R.; de Oliveira, Márcia C.C.; Cesarin-Sobrinho, Dari; Netto-Ferreira, José C.; Ferreira, Aurélio B.B. 2015. "Fluorescence and Docking Studies of the Interaction between Human Serum Albumin and Pheophytin." Molecules 20, no. 10: 19526-19539.