miRNA Stability in Frozen Plasma Samples
AbstractMicroRNAs (miRNAs) represent a family of small non-coding ribonucleic acids that post-transcriptionally inhibits the expression of their target messenger RNAs (mRNAs), thereby acting as general gene repressors. In this study we examined the relative quantity and stability of miRNA subjected to a long period of freezing; we compared the stability of eight miRNAs in the plasma of five human healthy controls before freezing and after six and 12 months of storage at −80 °C. In addition, we examined the plasma frozen for 14 years and the amount of miRNA still available. Using a Life Technologies protocol to amplify and quantify plasma miRNAs from EDTA (Ethylene Diamine Tetraacetic Acid)-treated blood, we analyzed the stability of eight miRNAs, (miR-125b-5p, miR-425-5p, miR-200b-5p, miR-200c-3p, miR-579-3p, miR-212-3p, miR-126-3p, and miR-21-5p). The miRNAs analyzed showed a high stability and long frozen half-life. View Full-Text
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Balzano, F.; Deiana, M.; Dei Giudici, S.; Oggiano, A.; Baralla, A.; Pasella, S.; Mannu, A.; Pescatori, M.; Porcu, B.; Fanciulli, G.; Zinellu, A.; Carru, C.; Deiana, L. miRNA Stability in Frozen Plasma Samples. Molecules 2015, 20, 19030-19040.
Balzano F, Deiana M, Dei Giudici S, Oggiano A, Baralla A, Pasella S, Mannu A, Pescatori M, Porcu B, Fanciulli G, Zinellu A, Carru C, Deiana L. miRNA Stability in Frozen Plasma Samples. Molecules. 2015; 20(10):19030-19040.Chicago/Turabian Style
Balzano, Francesca; Deiana, Marta; Dei Giudici, Silvia; Oggiano, Annalisa; Baralla, Angela; Pasella, Sara; Mannu, Andrea; Pescatori, Mario; Porcu, Baingio; Fanciulli, Giuseppe; Zinellu, Angelo; Carru, Ciriaco; Deiana, Luca. 2015. "miRNA Stability in Frozen Plasma Samples." Molecules 20, no. 10: 19030-19040.