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Molecules 2015, 20(10), 19030-19040; doi:10.3390/molecules201019030

miRNA Stability in Frozen Plasma Samples

1
Department of Biomedical Sciences, University of Sassari, vl. San Pietro 43b, Sassari 07100, Italy
2
Associazione “L’Isola dei Centenari”, Via Milano 4, Sassari 07100, Italy
3
Istituto Zooprofilattico Sperimentale della Sardegna, Via Vienna 2, Sassari 07100, Italy
4
Department of Clinical and Experimental Medicine, University of Sassari, vl. San Pietro 8, Sassari 07100, Italy
*
Author to whom correspondence should be addressed.
Academic Editor: Derek J. McPhee
Received: 24 July 2015 / Revised: 9 October 2015 / Accepted: 13 October 2015 / Published: 20 October 2015
(This article belongs to the Section Molecular Diversity)
View Full-Text   |   Download PDF [729 KB, uploaded 20 October 2015]   |  

Abstract

MicroRNAs (miRNAs) represent a family of small non-coding ribonucleic acids that post-transcriptionally inhibits the expression of their target messenger RNAs (mRNAs), thereby acting as general gene repressors. In this study we examined the relative quantity and stability of miRNA subjected to a long period of freezing; we compared the stability of eight miRNAs in the plasma of five human healthy controls before freezing and after six and 12 months of storage at −80 °C. In addition, we examined the plasma frozen for 14 years and the amount of miRNA still available. Using a Life Technologies protocol to amplify and quantify plasma miRNAs from EDTA (Ethylene Diamine Tetraacetic Acid)-treated blood, we analyzed the stability of eight miRNAs, (miR-125b-5p, miR-425-5p, miR-200b-5p, miR-200c-3p, miR-579-3p, miR-212-3p, miR-126-3p, and miR-21-5p). The miRNAs analyzed showed a high stability and long frozen half-life. View Full-Text
Keywords: miRNA; stability; long freezing times; sequences AU or UA miRNA; stability; long freezing times; sequences AU or UA
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

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MDPI and ACS Style

Balzano, F.; Deiana, M.; Dei Giudici, S.; Oggiano, A.; Baralla, A.; Pasella, S.; Mannu, A.; Pescatori, M.; Porcu, B.; Fanciulli, G.; Zinellu, A.; Carru, C.; Deiana, L. miRNA Stability in Frozen Plasma Samples. Molecules 2015, 20, 19030-19040.

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