Next Article in Journal
Study of the Biotransformation of Tongmai Formula by Human Intestinal Flora and Its Intestinal Permeability across the Caco-2 Cell Monolayer
Next Article in Special Issue
Phenolic Compounds from the Flowers of Bombax malabaricum and Their Antioxidant and Antiviral Activities
Previous Article in Journal
Resin-Immobilized Palladium Nanoparticle Catalysts for Organic Reactions in Aqueous Media: Morphological Aspects
Previous Article in Special Issue
Breeding Vegetables with Increased Content in Bioactive Phenolic Acids
Article Menu

Export Article

Open AccessArticle
Molecules 2015, 20(10), 18685-18703; doi:10.3390/molecules201018685

Antimicrobial Activity of Rhoeo discolor Phenolic Rich Extracts Determined by Flow Cytometry

1
Centro de Biotecnología FEMSA, Tecnologico de Monterrey, Campus Monterrey, Ave Eugenio Garza Sada 2501, Monterrey 64849, N.L., Mexico
2
Centro de Agrobiotecnología, Tecnologico de Monterrey, Campus Monterrey, Ave Eugenio Garza Sada 2501, Monterrey 64849, N.L., Mexico
3
Centro de Innovación y Transferida en Salud, Escuela de Medicina, Tecnologico de Monterrey, Campus Monterrey, Ave Morones Prieto No. 3000 Pte., Col. Los Doctores, C.P. Monterrey 64710, N.L., Mexico
*
Author to whom correspondence should be addressed.
Academic Editor: Luis Cisneros-Zevallos
Received: 5 August 2015 / Revised: 7 September 2015 / Accepted: 18 September 2015 / Published: 14 October 2015
(This article belongs to the Special Issue Recent Advances in Plant Phenolics)
View Full-Text   |   Download PDF [2836 KB, uploaded 14 October 2015]   |  

Abstract

Traditional medicine has led to the discovery of important active substances used in several health-related areas. Phytochemicals in Rhoeo discolor extracts have proven to have important antimicrobial activity. In the present study, our group determined the antimicrobial effects of extracts of Rhoeo discolor, a plant commonly used in Mexico for both medicinal and ornamental purposes. We evaluated the in vitro activity of phenolic rich extracts against specifically chosen microorganisms of human health importance by measuring their susceptibility via agar-disc diffusion assay and flow cytometry: Gram-positive Listeria innocua and Streptococcus mutans, Gram-negative Escherichia coli and Pseudomonas aeruginosa, and lastly a fungal pathogen Candida albicans. Ten different extracts were tested in eight different doses on all the microorganisms. Analytical data revealed a high content of phenolic compounds. Both agar-disc diffusion assay and flow cytometry results demonstrated that Pseudomonas aeruginosa was the least affected by extract exposure. However, low doses of these extracts (predominantly polar), in a range from 1 to 4 μg/mL, did produce a statistically significant bacteriostatic and bactericidal effect on the rest of the microorganisms. These results suggest the addition of certain natural extracts from Rhoeo discolor could act as antibacterial and antimycotic drugs or additives for foods and cosmetics. View Full-Text
Keywords: antifungal; antimicrobial; Candida albicans; Escherichia coli; flow cytometry; Listeria innocua; phenolic compounds; Pseudomonas aeruginosa; Rhoeo discolor; Streptococcus mutans antifungal; antimicrobial; Candida albicans; Escherichia coli; flow cytometry; Listeria innocua; phenolic compounds; Pseudomonas aeruginosa; Rhoeo discolor; Streptococcus mutans
Figures

Figure 1

This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

Supplementary material

Scifeed alert for new publications

Never miss any articles matching your research from any publisher
  • Get alerts for new papers matching your research
  • Find out the new papers from selected authors
  • Updated daily for 49'000+ journals and 6000+ publishers
  • Define your Scifeed now

SciFeed Share & Cite This Article

MDPI and ACS Style

García-Varela, R.; García-García, R.M.; Barba-Dávila, B.A.; Fajardo-Ramírez, O.R.; Serna-Saldívar, S.O.; Cardineau, G.A. Antimicrobial Activity of Rhoeo discolor Phenolic Rich Extracts Determined by Flow Cytometry. Molecules 2015, 20, 18685-18703.

Show more citation formats Show less citations formats

Related Articles

Article Metrics

Article Access Statistics

1

Comments

[Return to top]

Molecules EISSN 1420-3049 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert
Back to Top