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Molecules 2014, 19(7), 10635-10649; doi:10.3390/molecules190710635

Effects of Echium plantagineum L. Bee Pollen on Basophil Degranulation: Relationship with Metabolic Profile

REQUIMTE/Laboratório de Farmacognosia, Departamento de Química, Faculdade de Farmácia, Universidade do Porto, Rua de Jorge Viterbo Ferreira nº 228, 4050-313 Porto, Portugal
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Received: 7 May 2014 / Revised: 10 July 2014 / Accepted: 14 July 2014 / Published: 22 July 2014
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Abstract

This study aimed to evaluate the anti-allergic potential of Echium plantagineum L. bee pollen and to characterize its primary metabolites. The activity of E. plantagineum hydromethanolic extract, devoid of alkaloids, was tested against β-hexosaminidase release in rat basophilic leukemic cells (RBL-2H3). Two different stimuli were used: calcium ionophore A23187 and IgE/antigen. Lipoxygenase inhibitory activity was evaluated in a cell-free system using soybean lipoxygenase. Additionally, the extract was analysed by HPLC-UV for organic acids and by GC-IT/MS for fatty acids. In RBL-2H3 cells stimulated either with calcium ionophore or IgE/antigen, the hydromethanolic extract significantly decreased β-hexosaminidase release until the concentration of 2.08 mg/mL, without compromising cellular viability. No effect was found on lipoxygenase. Concerning extract composition, eight organic acids and five fatty acids were determined for the first time. Malonic acid (80%) and α-linolenic acid (27%) were the main compounds in each class. Overall, this study shows promising results, substantiating for the first time the utility of intake of E. plantagineum bee pollen to prevent allergy and ameliorate allergy symptoms, although a potentiation of an allergic response can occur, depending on the dose used. View Full-Text
Keywords: Echium plantagineum L. bee pollen; RBL-2H3 cells degranulation; β-hexosaminidase; lipoxygenase; organic acids; fatty acids Echium plantagineum L. bee pollen; RBL-2H3 cells degranulation; β-hexosaminidase; lipoxygenase; organic acids; fatty acids
This is an open access article distributed under the Creative Commons Attribution License (CC BY 3.0).

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MDPI and ACS Style

Moita, E.; Sousa, C.; Andrade, P.B.; Fernandes, F.; Pinho, B.R.; Silva, L.R.; Valentão, P. Effects of Echium plantagineum L. Bee Pollen on Basophil Degranulation: Relationship with Metabolic Profile. Molecules 2014, 19, 10635-10649.

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