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Molecules 2014, 19(7), 10427-10439; doi:10.3390/molecules190710427

Qualitative and Quantitative Analysis of Rhizoma Smilacis glabrae by Ultra High Performance Liquid Chromatography Coupled with LTQ OrbitrapXL Hybrid Mass Spectrometry

1
The Second College of Clinic Medicine, Guangzhou University of Chinese Medicine, Guangzhou 510000, China
2
Guangdong Provincial Hospital of Chinese Medicine, Guangzhou 510000, China
*
Author to whom correspondence should be addressed.
Received: 3 June 2014 / Revised: 10 July 2014 / Accepted: 11 July 2014 / Published: 17 July 2014
(This article belongs to the Section Natural Products)
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Abstract

Rhizoma Smilacis glabrae, a traditional Chinese medicine (TCM) as well as a functional food, has been commonly used for detoxification treatments, relieving dampness and as a diuretic. In order to quickly define the chemical profiles and control the quality of Smilacis glabrae, ultra high performance liquid chromatography coupled with electrospray ionization hybrid linear trap quadrupole orbitrap mass spectrometry (UHPLC-ESI/LTQ-Orbitrap-MS) was applied for simultaneous identification and quantification of its bioactive constituents. A total of 56 compounds, including six new compounds, were identified or tentatively deduced on the basis of their retention behaviors, mass spectra, or by comparison with reference substances and literature data. The identified compounds belonged to flavonoids, phenolic acids and phenylpropanoid glycosides. In addition, an optimized UHPLC-ESI/LTQ-Orbitrap-MS method was established for quantitative determination of six marker compounds from five batches. The validation of the method, including linearity, sensitivity (LOQ), precision, repeatability and spike recoveries, was carried out and demonstrated to be satisfied the requirements of quantitative analysis. The results suggested that the established method would be a powerful and reliable analytical tool for the characterization of multi-constituent in complex chemical system and quality control of TCM. View Full-Text
Keywords: Rhizoma Smilacis glabrae; UHPLC-ESI/LTQ-Orbitrap-MS; qualitative analysis; quantitative analysis Rhizoma Smilacis glabrae; UHPLC-ESI/LTQ-Orbitrap-MS; qualitative analysis; quantitative analysis
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MDPI and ACS Style

Chen, S.-D.; Lu, C.-J.; Zhao, R.-Z. Qualitative and Quantitative Analysis of Rhizoma Smilacis glabrae by Ultra High Performance Liquid Chromatography Coupled with LTQ OrbitrapXL Hybrid Mass Spectrometry. Molecules 2014, 19, 10427-10439.

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