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Molecules 2014, 19(6), 8503-8517; doi:10.3390/molecules19068503

Dehydroabietic Acid Isolated from Commiphora opobalsamum Causes Endothelium-Dependent Relaxation of Pulmonary Artery via PI3K/Akt-eNOS Signaling Pathway

1
College of Pharmacy, Harbin Medical University-Daqing, Daqing 163319, Heilongjiang, China
2
Biopharmaceutical Institute of the Heilongjiang Academy of Medical Sciences, Harbin 150081, Heilongjiang, China
3
College of Pharmacy, Harbin University of Commerce, Harbin 150028, Heilongjiang, China
*
Author to whom correspondence should be addressed.
Received: 12 March 2014 / Revised: 16 June 2014 / Accepted: 17 June 2014 / Published: 23 June 2014
(This article belongs to the Section Natural Products)
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Abstract

Commiphora opobalsamum is a Traditional Chinese Medicine used to treat traumatic injury, mainly by relaxing blood vessels. In this study, two diterpenes, dehydroabietic acid (DA) and sandaracopimaric acid (SA) were obtained from it by a bioassay-guided approach using isolated rat pulmonary artery rings. The structures of the two compounds were elucidated by spectroscopic methods (IR, 1H- and 13C-NMR, HR-ESI-MS). Both DA and SA reduced the contraction of phenylephrine-induced pulmonary arteries in a concentration-dependent manner, and endothelium contributed greatly to the vasodilatory effect of DA. This effect of DA was attenuated by NG-Nitro-L-arginine methyl ester (L-NAME, an eNOS inhibitor). Meanwhile, DA increased nitric oxide (NO) production, along with the increase of phosphorylation level of eNOS and Akt in endothelial cells. LY294002 (a PI3K inhibitor) could reverse this effect, which suggested the endothelial PI3K/Akt pathway involved in the mechanism underlying DA-induced relaxation of pulmonary artery. This work provided evidence of vasorelaxant substances in Commiphora opobalsamum and validated that PI3K/Akt-eNOS pathway was associated with DA-induced pulmonary artery vasodilation. View Full-Text
Keywords: Commiphora opobalsamum; dehydroabietic acid; sandaracopimaric acid; nitric oxide; vasodilation; pulmonary artery Commiphora opobalsamum; dehydroabietic acid; sandaracopimaric acid; nitric oxide; vasodilation; pulmonary artery
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This is an open access article distributed under the Creative Commons Attribution License (CC BY 3.0).

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MDPI and ACS Style

Gao, W.; Dong, X.; Xie, N.; Zhou, C.; Fan, Y.; Chen, G.; Wang, Y.; Wei, T.; Zhu, D. Dehydroabietic Acid Isolated from Commiphora opobalsamum Causes Endothelium-Dependent Relaxation of Pulmonary Artery via PI3K/Akt-eNOS Signaling Pathway. Molecules 2014, 19, 8503-8517.

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