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Molecules 2014, 19(6), 7901-7912; doi:10.3390/molecules19067901

Enhancement of Phenolics, Resveratrol and Antioxidant Activity by Nitrogen Enrichment in Cell Suspension Culture of Vitis vinifera

School of Bioresources and Technology, King Mongkut's University of Technology Thonburi, 49 Teintalay Rd., Thakam, Bangkhuntein, 10150 Bangkok, Thailand
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Received: 21 April 2014 / Revised: 3 June 2014 / Accepted: 4 June 2014 / Published: 11 June 2014
(This article belongs to the Section Natural Products)
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Abstract

Ammonium nitrate (NH4NO3), an important nitrogen source (34% N), has been used as an elicitor to stimulate plant growth and development as well as induce secondary metabolites under controlled conditions. In the present paper, we investigated the enhancement of cell biomass, total phenolics, resveratrol levels, and antioxidant activity of Vitis vinifera cv. Pok Dum by nitrogen enrichment (MS medium supplemented with NH4NO3 at 0, 500, 1,000, 5,000 and 10,000 mg/L). The highest accumulations of biomass, phenolics and resveratrol contents were observed at 8.8-fold (86.6 g DW/L), 15.9-fold (71.91 mg GAE/g DW) and 5.6-fold (277.89 µg/g DW) by the 14th day, in the medium supplemented with 500 mg/L NH4NO3. Moreover, the antioxidant activities of cultured grape cells estimated by the DPPH· and ABTS·+ assay were positively correlated with phenolics and resveratrol, and the maximum activity was also observed in cultured cells with 500 mg/L NH4NO3 at 176.11 and 267.79 mmol TE/100 g DW, respectively. View Full-Text
Keywords: phytochemical; ammonium nitrate; antioxidative activity; trans-resveratrol phytochemical; ammonium nitrate; antioxidative activity; trans-resveratrol
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MDPI and ACS Style

Sae-Lee, N.; Kerdchoechuen, O.; Laohakunjit, N. Enhancement of Phenolics, Resveratrol and Antioxidant Activity by Nitrogen Enrichment in Cell Suspension Culture of Vitis vinifera. Molecules 2014, 19, 7901-7912.

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