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Molecules 2014, 19(6), 7255-7268; doi:10.3390/molecules19067255

A Novel Phage-Library-Selected Peptide Inhibits Human TNF-α Binding to Its Receptors

1
Department of Medical Biotechnologies, University of Siena, Via A. Moro 2, 53100 Siena, Italy
2
SetLance srl, via Fiorentina 1, 53100 Siena, Italy
3
Laboratorio di Patologia Clinica, Azienda Ospedaliera Universitaria Senese, 53100 Siena, Italy
*
Author to whom correspondence should be addressed.
Received: 10 April 2014 / Revised: 22 May 2014 / Accepted: 27 May 2014 / Published: 3 June 2014
(This article belongs to the Special Issue Peptide Chemistry)
View Full-Text   |   Download PDF [1315 KB, uploaded 18 June 2014]   |  

Abstract

We report the identification of a new human tumor necrosis factor-alpha (TNF-α) specific peptide selected by competitive panning of a phage library. Competitive elution of phages was obtained using the monoclonal antibody adalimumab, which neutralizes pro-inflammatory processes caused by over-production of TNF-α in vivo, and is used to treat severe symptoms of rheumatoid arthritis. The selected peptide was synthesized in monomeric and branched form and analyzed for binding to TNF-α and competition with adalimumab and TNF-α receptors. Results of competition with TNF-α receptors in surface plasmon resonance and melanoma cells expressing both TNF receptors make the peptide a candidate compound for the development of a novel anti-TNF-α drug. View Full-Text
Keywords: TNF-α; anti-TNF-α peptide; branched peptides; phage display; solid-phase synthesis; competitive selection; surface plasmon resonance TNF-α; anti-TNF-α peptide; branched peptides; phage display; solid-phase synthesis; competitive selection; surface plasmon resonance
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This is an open access article distributed under the Creative Commons Attribution License (CC BY 3.0).

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MDPI and ACS Style

Brunetti, J.; Lelli, B.; Scali, S.; Falciani, C.; Bracci, L.; Pini, A. A Novel Phage-Library-Selected Peptide Inhibits Human TNF-α Binding to Its Receptors. Molecules 2014, 19, 7255-7268.

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