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Molecules 2014, 19(4), 4409-4417; doi:10.3390/molecules19044409
Article

HPLC-DPPH Screening Method for Evaluation of Antioxidant Compounds Extracted from Semen Oroxyli

1
, 1
 and 1,2,*
Received: 5 March 2014; in revised form: 2 April 2014 / Accepted: 3 April 2014 / Published: 10 April 2014
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Abstract: Semen Oroxyli, derived from the seed of Oroxylum indicum L., is a commonly used Traditional Chinese Medicine with beneficial effects against several respiratory disorders. Antioxidative flavonoids may be partly responsible for its medicinal functions. The aim of this study was to rapidly determine the antioxidants in Semen Oroxyli based on a HPLC-DPPH method. Four major flavonoids, baicalein-7-O-gentiobioside, baicalein-7-O-glucoside, baicalein, and baicalin, were identified as the active components against DPPH free radicals, which is in accord with the results of our former traditional activity-guided phytochemical study. The oxidative products of the four antioxidant flavonoids were studied in the DPPH spiking HPLC assay, it was suggested that the three active flavonoid glycosides were converted into 5,6-dihydroxy-7-methoxyflavone, which implied that an additional hydroxyl at C-6 in 5,7-dihydroxyflavones plays an important role in the DPPH assay.
Keywords: Oroxylum indicum; flavonoid; HPLC-DPPH; antioxidant; rapid screening Oroxylum indicum; flavonoid; HPLC-DPPH; antioxidant; rapid screening
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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MDPI and ACS Style

Yan, R.; Cao, Y.; Yang, B. HPLC-DPPH Screening Method for Evaluation of Antioxidant Compounds Extracted from Semen Oroxyli. Molecules 2014, 19, 4409-4417.

AMA Style

Yan R, Cao Y, Yang B. HPLC-DPPH Screening Method for Evaluation of Antioxidant Compounds Extracted from Semen Oroxyli. Molecules. 2014; 19(4):4409-4417.

Chicago/Turabian Style

Yan, Renyi; Cao, Yangyang; Yang, Bin. 2014. "HPLC-DPPH Screening Method for Evaluation of Antioxidant Compounds Extracted from Semen Oroxyli." Molecules 19, no. 4: 4409-4417.


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