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Molecules 2014, 19(4), 4105-4114; doi:10.3390/molecules19044105
Article

Fluorescent Probes for Insect Ryanodine Receptors: Candidate Anthranilic Diamides

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Received: 16 January 2014; in revised form: 13 March 2014 / Accepted: 21 March 2014 / Published: 2 April 2014
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Abstract: Diamide insecticides with high efficacy against pests and good environmental safety are broadly applied in crop protection. They act at a poorly-defined site in the very complex ryanodine (Ry) receptor (RyR) potentially accessible to a fluorescent probe. Two N-propynyl analogs of the major anthranilic diamide insecticides chlorantraniliprole (Chlo) and cyantraniliprole (Cyan) were accordingly synthesized and converted into two fluorescent ligands by click reaction coupling with 3-azido-7-hydroxy-2H-chromen-2-one. The new diamide analogs and fluorescent ligands were shown to be nearly as potent as Chlo and Cyan in inhibition of [3H]Chlo binding and stimulation of [3H]Ry binding in house fly thoracic muscle RyR. Although the newly synthesized compounds had only moderate activity in insect larvicidal activity assays, their high in vitro potency in a validated insect RyR binding assay encourages further development of fluorescent probes for insect RyRs.
Keywords: fluorescent probe; affinity; ryanodine receptor fluorescent probe; affinity; ryanodine receptor
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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MDPI and ACS Style

Wang, Y.; Guo, L.; Qi, S.; Zhang, H.; Liu, K.; Liu, R.; Liang, P.; Casida, J.E.; Liu, S. Fluorescent Probes for Insect Ryanodine Receptors: Candidate Anthranilic Diamides. Molecules 2014, 19, 4105-4114.

AMA Style

Wang Y, Guo L, Qi S, Zhang H, Liu K, Liu R, Liang P, Casida JE, Liu S. Fluorescent Probes for Insect Ryanodine Receptors: Candidate Anthranilic Diamides. Molecules. 2014; 19(4):4105-4114.

Chicago/Turabian Style

Wang, Yi; Guo, Lei; Qi, Suzhen; Zhang, Hao; Liu, Kechang; Liu, Ruiquan; Liang, Pei; Casida, John E.; Liu, Shangzhong. 2014. "Fluorescent Probes for Insect Ryanodine Receptors: Candidate Anthranilic Diamides." Molecules 19, no. 4: 4105-4114.



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