Next Article in Journal
Previous Article in Journal
Previous Article in Special Issue
Molecules 2014, 19(3), 3460-3470; doi:10.3390/molecules19033460
Article

Metabonomic Analysis of Water Extracts from Different Angelica Roots by 1H-Nuclear Magnetic Resonance Spectroscopy

1,2
, 2
, 3
, 1
, 3
, 2
 and 1,4,*
Received: 20 December 2013; in revised form: 25 February 2014 / Accepted: 13 March 2014 / Published: 20 March 2014
(This article belongs to the Special Issue Phytochemicals: Analytical and Medicinal Chemistry)
View Full-Text   |   Download PDF [1538 KB, uploaded 18 June 2014]
Abstract: Angelica Radix, the roots of the genus Angelica, has been used for more than 2,000 years as a traditional medicine in Eastern Asia. The Chinese Pharmacopoeia records more than 100 herbal formulae containing Angelica roots. There are two common sources of Angelica roots, Angelica sinensis from China and A. gigas from Korea. The two species of Angelica roots differ in their chemical compositions, pharmacological properties and clinical efficacy. 1H-NMR metabolic profiling has recently emerged as a promising quality control method for food and herbal chemistry. We explored the use of 1H-NMR metabolic profiling for the quality control of Angelica Radix. Unlike previous work, we performed the metabolic profiling on hot water extracts, so as to mimic the clinically relevant preparation method. Unsupervised principle component analyses of both the full spectral profile and a selection of targeted molecules revealed a clear differentiation of three types of Angelica roots. In addition, the levels of 13 common metabolites were measured. Statistically significant differences in the levels of glucose, fructose and threonine were found between different sources of Angelica. Ferulic acid, a marker commonly used to evaluate Angelica root, was detected in our samples, but the difference in ferulic acid levels between the samples was not statistically significant. Overall, we successfully applied 1H-NMR metabolic profiling with water extraction to discriminate all three sources of Angelica roots, and obtained quantitative information of many common metabolites.
Keywords: Angelica sinensis; Angelica gigas; Traditional Chinese Medicine; NMR; metabonomics; metabolomics Angelica sinensis; Angelica gigas; Traditional Chinese Medicine; NMR; metabonomics; metabolomics
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Export to BibTeX |
EndNote


MDPI and ACS Style

Chan, P.H.; Zhang, W.L.; Lau, C.-H.; Cheung, C.Y.; Keun, H.C.; Tsim, K.W.K.; Lam, H. Metabonomic Analysis of Water Extracts from Different Angelica Roots by 1H-Nuclear Magnetic Resonance Spectroscopy. Molecules 2014, 19, 3460-3470.

AMA Style

Chan PH, Zhang WL, Lau C-H, Cheung CY, Keun HC, Tsim KWK, Lam H. Metabonomic Analysis of Water Extracts from Different Angelica Roots by 1H-Nuclear Magnetic Resonance Spectroscopy. Molecules. 2014; 19(3):3460-3470.

Chicago/Turabian Style

Chan, Pui H.; Zhang, Wendy L.; Lau, Chung-Ho; Cheung, Chi Y.; Keun, Hector C.; Tsim, Karl W.K.; Lam, Henry. 2014. "Metabonomic Analysis of Water Extracts from Different Angelica Roots by 1H-Nuclear Magnetic Resonance Spectroscopy." Molecules 19, no. 3: 3460-3470.


Molecules EISSN 1420-3049 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert