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Molecules 2014, 19(12), 20675-20694; doi:10.3390/molecules191220675

Synergistic Radiation Protective Effect of Purified Auricularia auricular-judae Polysaccharide (AAP IV) with Grape Seed Procyanidins

1
School of Food Science and Engineering, Harbin Institute of Technology, 73 HuangHe Road, NanGang District, Harbin 150090, China
2
School of Forestry, Northeast Forestry University, 26 HeXing Road, DongLi District, Harbin 150040, China
3
Department of Food Science, Cornell University, Ithaca, NY 14853, USA
*
Authors to whom correspondence should be addressed.
Received: 3 November 2014 / Revised: 27 November 2014 / Accepted: 2 December 2014 / Published: 11 December 2014
(This article belongs to the Section Natural Products)
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Abstract

The aim of this study was to investigate the synergistic antioxidant potential and protective effect of grape seed procyanidins (GSP) in combination with Auricularia auricular-judae polysaccharides (AAP IV) on radiation injury in splenocytes. Rat splenocyte irradiation resulted in significantly higher apoptosis rate, malondialdehyde (MDA) (p < 0.005), reactive oxygen species (ROS) (p < 0.01); cell viability, total superoxide dismutase (T-SOD) (p < 0.01), catalase (CAT) (p < 0.01), glutathione peroxidase (GSH-PX) (p < 0.05), activity and glutathione (GSH) (p < 0.01) levels were significantly reduced, compared with the control group. “GSP + AAP IV” treatment of rat splenocytes at doses of “GSP (0.3 μg/mL) + AAP IV (50 μg/mL)” displayed higher radioprotective and antioxidative effects than the administration of either GSP or AAP IV, as evident by lower levels of MDA (p < 0.001) concentration, as well as higher cell viability and T-SOD (p < 0.05), CAT (p < 0.005), GSH-PX (p < 0.01) and GSH content compared to the radiation group. In addition, in vivo studies have shown that “GSP + AAP IV” significantly ameliorated the decrease of spleen index (p < 0.005) and spleen GSH (p < 0.005) levels and significantly inhibited the increase of MDA (p < 0.005) levels of spleen with radiation-induced damage, compared with the non-treated group. The in vivo and in vitro results suggested that GSP and AAP IV have a synergistic protective effect against radiation-induced injury by improving the antioxidant and immunomodulation activities. View Full-Text
Keywords: grape seed procyanidins; Auricularia auricular-judae polysaccharides; radiation protective; synergistic; splenocytes grape seed procyanidins; Auricularia auricular-judae polysaccharides; radiation protective; synergistic; splenocytes
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

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MDPI and ACS Style

Bai, H.; Wang, Z.; Cui, J.; Yun, K.; Zhang, H.; Liu, R.H.; Fan, Z.; Cheng, C. Synergistic Radiation Protective Effect of Purified Auricularia auricular-judae Polysaccharide (AAP IV) with Grape Seed Procyanidins. Molecules 2014, 19, 20675-20694.

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