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Molecules 2014, 19(10), 16416-16427; doi:10.3390/molecules191016416

Optimization of Extraction Conditions of Areca Seed Polyphenols and Evaluation of Their Antioxidant Activities

1
College of Food Science, Hainan University, Haikou 570228, China
2
Jiangsu Academy of Agricultural Sciences, Nanjing 210014, China
*
Authors to whom correspondence should be addressed.
Received: 21 August 2014 / Revised: 9 October 2014 / Accepted: 9 October 2014 / Published: 13 October 2014
(This article belongs to the Section Natural Products)
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Abstract

Polyphenols are functional compounds in plants, which possess many bioactivities beneficial for humans. The aim of this study was to establish a highly efficient method for extracting polyphenol compounds from areca seeds and further to identify polyphenols and antioxidant properties of the seeds. A quadratic general rotary unitized design was used to determine the optimal extraction process. The polyphenols were identified using LC-TOF-MS. By comparison with ascorbic acid (Vc), the antioxidant activities of the ethanol extracts were evaluated using three complementary in vitro assays: inhibition of the DPPH (1,1-diphenyl-2-picrylhydrazyl) radical-scavenging activity, hydroxyl radical-scavenging activity, and reducing ability. The two major polyphenols obtained were epicatechin and syringic acid. The ethanol extracts of areca seeds showed significantly greater antioxidant activity (p < 0.05) than Vc using the DPPH and reducing power assay, but lower ability (p < 0.05) using the hydroxyl radical assay. The results indicate that the areca seed is an excellent food material with potential antioxidant properties. View Full-Text
Keywords: antioxidant activities; Areca catechu L.; extraction; polyphenols antioxidant activities; Areca catechu L.; extraction; polyphenols
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

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Zhang, W.-M.; Huang, W.-Y.; Chen, W.-X.; Han, L.; Zhang, H.-D. Optimization of Extraction Conditions of Areca Seed Polyphenols and Evaluation of Their Antioxidant Activities. Molecules 2014, 19, 16416-16427.

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