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Molecules 2014, 19(10), 16001-16012; doi:10.3390/molecules191016001

Efficient Biotransformation of Polysialogangliosides for Preparation of GM1 by Cellulosimicrobium sp. 21

1
China-Japan Union Hospital, Jilin University, Changchun 130033, Jilin, China
2
School of Life Sciences, Northeast Normal University, Changchun 130024, Jilin, China
These authors contributed equally to this work.
*
Authors to whom correspondence should be addressed.
Received: 1 September 2014 / Revised: 28 September 2014 / Accepted: 29 September 2014 / Published: 8 October 2014
(This article belongs to the Section Metabolites)
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Abstract

A new ganglioside transformed strain isolated from soil was identified as Cellulosimicrobium sp. 21. It produced a sialidase which transformed polysialo-gangliosides GD1 and GT1 into a monosialoterahexosylganglioside, i.e., ganglioside GM1. The sialidase had both NeuAc-α-2,3- and NeuAc-α-2,8-sialidase activity without producing asiolo-GM1. The optimum conditions were evaluated and it was found that the transformation was optimally performed at 30 °C and pH 7.0. The substrate should be added at the beginning of the reaction and the concentration of substrate was 3% (w/v). Under these optimum conditions, Cellulosimicrobium sp. 21 converted GD1 and GT1 into GM1 in inorganic medium in a 5 L bioreactor with the recovery rate of 69.3%. The product contained 50.3% GM1 and was purified on silica to give the product with 95% of GM1 with a recovery rate of 30.5%. Therefore, Cellulosimicrobium sp. 21 has potential to be applied in the production of GM1 in the pharmaceutical industry. View Full-Text
Keywords: polysialoganglioside; Cellulosimicrobium sp. 21; biotransformation; sialidase; GM1 polysialoganglioside; Cellulosimicrobium sp. 21; biotransformation; sialidase; GM1
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

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MDPI and ACS Style

Zheng, Y.; Ji, L.; Leng, J.; Yuan, Y.; Chen, H.; Gou, D.; Gao, Y.; Zhou, Y. Efficient Biotransformation of Polysialogangliosides for Preparation of GM1 by Cellulosimicrobium sp. 21. Molecules 2014, 19, 16001-16012.

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