Structural Characterization of Intrinsically Disordered Proteins by NMR Spectroscopy
AbstractRecent advances in NMR methodology and techniques allow the structural investigation of biomolecules of increasing size with atomic resolution. NMR spectroscopy is especially well-suited for the study of intrinsically disordered proteins (IDPs) and intrinsically disordered regions (IDRs) which are in general highly flexible and do not have a well-defined secondary or tertiary structure under functional conditions. In the last decade, the important role of IDPs in many essential cellular processes has become more evident as the lack of a stable tertiary structure of many protagonists in signal transduction, transcription regulation and cell-cycle regulation has been discovered. The growing demand for structural data of IDPs required the development and adaption of methods such as 13C-direct detected experiments, paramagnetic relaxation enhancements (PREs) or residual dipolar couplings (RDCs) for the study of ‘unstructured’ molecules in vitro and in-cell. The information obtained by NMR can be processed with novel computational tools to generate conformational ensembles that visualize the conformations IDPs sample under functional conditions. Here, we address NMR experiments and strategies that enable the generation of detailed structural models of IDPs. View Full-Text
Scifeed alert for new publicationsNever miss any articles matching your research from any publisher
- Get alerts for new papers matching your research
- Find out the new papers from selected authors
- Updated daily for 49'000+ journals and 6000+ publishers
- Define your Scifeed now
Kosol, S.; Contreras-Martos, S.; Cedeño, C.; Tompa, P. Structural Characterization of Intrinsically Disordered Proteins by NMR Spectroscopy. Molecules 2013, 18, 10802-10828.
Kosol S, Contreras-Martos S, Cedeño C, Tompa P. Structural Characterization of Intrinsically Disordered Proteins by NMR Spectroscopy. Molecules. 2013; 18(9):10802-10828.Chicago/Turabian Style
Kosol, Simone; Contreras-Martos, Sara; Cedeño, Cesyen; Tompa, Peter. 2013. "Structural Characterization of Intrinsically Disordered Proteins by NMR Spectroscopy." Molecules 18, no. 9: 10802-10828.