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Molecules 2013, 18(9), 10629-10638; doi:10.3390/molecules180910629
Article

Pancreatic Lipase Inhibitory Gallotannins from Galla Rhois with Inhibitory Effects on Adipocyte Differentiation in 3T3-L1 Cells

1,†
, 2,†
, 2
, 3
, 4
, 5
 and 6,*
1 Daegyeong Institute for Regional Program Evaluation, Gyeongsan 712-210, Korea 2 College of Pharmacy, Research Institute of Pharmaceutical Sciences, Kyungpook National University, Daegu 702-701, Korea 3 Department of Food Biotechnology, Andong National University, Andong 760-749, Korea 4 Department of Life Science and Biotechnology, Dongeui University, Busan 614-714, Korea 5 Faculty of Health and Welfare Sciences, Okayama Prefectural University, Okayama 719-1197, Japan 6 Department of Herbal Medicinal Pharmacology, Daegu Haany University, Gyeongsan 712-715, Korea These authors equally contributed to this work.
* Author to whom correspondence should be addressed.
Received: 21 June 2013 / Revised: 26 August 2013 / Accepted: 26 August 2013 / Published: 2 September 2013
(This article belongs to the Section Metabolites)
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Abstract

Activity-guided isolation of a methanolic extract of Galla Rhois using pancreatic lipase and 3T3-L1 adipocytes led to the isolation of seven phenolic compounds: protoaphin-fb (1), 2-O-digalloyl-1,3,4,6-tetra-O-galloyl-b-D-glucose (2), 1,2,3,4,6-penta-O-galloyl-b-D-glucose (3), 1,2,4,6-tetra-O-galloyl-b-D-glucose (4), 3-hydroxy-5-methoxy-phenol 1-O-b-D-glucoside (5), methylgallate (6), and gallic acid (7). Their structures were established on the basis of NMR and MS spectroscopic data interpretation. All isolates were evaluated for their inhibitory effects on pancreatic lipase, and compounds 1-5 exhibited potent inhibitory effects on this enzyme, with IC50 values ranging from 30.6 ± 2.4 to 3.5 ± 0.5 mM. In addition, the highly galloylated compound 2 was also found to induce potent inhibition of adipocyte differentiation in 3T3-L1 cells.
Keywords: Galla Rhois; gallotannins; pancreatic lipase; protoaphin-fb; 3T3-L1; 2-O-digalloyl-1,3,4,6-tetra-O-galloyl-b-D-glucose Galla Rhois; gallotannins; pancreatic lipase; protoaphin-fb; 3T3-L1; 2-O-digalloyl-1,3,4,6-tetra-O-galloyl-b-D-glucose
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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MDPI and ACS Style

Kwon, O.J.; Bae, J.-S.; Lee, H.Y.; Hwang, J.-Y.; Lee, E.-W.; Ito, H.; Kim, T.H. Pancreatic Lipase Inhibitory Gallotannins from Galla Rhois with Inhibitory Effects on Adipocyte Differentiation in 3T3-L1 Cells. Molecules 2013, 18, 10629-10638.

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