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Pancreatic Lipase Inhibitory Gallotannins from Galla Rhois with Inhibitory Effects on Adipocyte Differentiation in 3T3-L1 Cells
Daegyeong Institute for Regional Program Evaluation, Gyeongsan 712-210, Korea
College of Pharmacy, Research Institute of Pharmaceutical Sciences, Kyungpook National University, Daegu 702-701, Korea
Department of Food Biotechnology, Andong National University, Andong 760-749, Korea
Department of Life Science and Biotechnology, Dongeui University, Busan 614-714, Korea
Faculty of Health and Welfare Sciences, Okayama Prefectural University, Okayama 719-1197, Japan
Department of Herbal Medicinal Pharmacology, Daegu Haany University, Gyeongsan 712-715, Korea
These authors equally contributed to this work.
* Author to whom correspondence should be addressed.
Received: 21 June 2013; in revised form: 26 August 2013 / Accepted: 26 August 2013 / Published: 2 September 2013
Abstract: Activity-guided isolation of a methanolic extract of Galla Rhois using pancreatic lipase and 3T3-L1 adipocytes led to the isolation of seven phenolic compounds: protoaphin-fb (1), 2-O-digalloyl-1,3,4,6-tetra-O-galloyl-b-D-glucose (2), 1,2,3,4,6-penta-O-galloyl-b-D-glucose (3), 1,2,4,6-tetra-O-galloyl-b-D-glucose (4), 3-hydroxy-5-methoxy-phenol 1-O-b-D-glucoside (5), methylgallate (6), and gallic acid (7). Their structures were established on the basis of NMR and MS spectroscopic data interpretation. All isolates were evaluated for their inhibitory effects on pancreatic lipase, and compounds 1-5 exhibited potent inhibitory effects on this enzyme, with IC50 values ranging from 30.6 ± 2.4 to 3.5 ± 0.5 mM. In addition, the highly galloylated compound 2 was also found to induce potent inhibition of adipocyte differentiation in 3T3-L1 cells.
Keywords: Galla Rhois; gallotannins; pancreatic lipase; protoaphin-fb; 3T3-L1; 2-O-digalloyl-1,3,4,6-tetra-O-galloyl-b-D-glucose
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Kwon, O.J.; Bae, J.-S.; Lee, H.Y.; Hwang, J.-Y.; Lee, E.-W.; Ito, H.; Kim, T.H. Pancreatic Lipase Inhibitory Gallotannins from Galla Rhois with Inhibitory Effects on Adipocyte Differentiation in 3T3-L1 Cells. Molecules 2013, 18, 10629-10638.
Kwon OJ, Bae J-S, Lee HY, Hwang J-Y, Lee E-W, Ito H, Kim TH. Pancreatic Lipase Inhibitory Gallotannins from Galla Rhois with Inhibitory Effects on Adipocyte Differentiation in 3T3-L1 Cells. Molecules. 2013; 18(9):10629-10638.
Kwon, O Jun; Bae, Jong-Sup; Lee, Ha Y.; Hwang, Ju-Young; Lee, Eun-Woo; Ito, Hideyuki; Kim, Tae H. 2013. "Pancreatic Lipase Inhibitory Gallotannins from Galla Rhois with Inhibitory Effects on Adipocyte Differentiation in 3T3-L1 Cells." Molecules 18, no. 9: 10629-10638.