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Isoliquiritigen Enhances the Antitumour Activity and Decreases the Genotoxic Effect of Cyclophosphamide
School of Pharmacy, Shihezi University, Shihezi 832002, China
Lanzhou University Second Hospital, Lanzhou University, Lanzhou 730000, China
School of integrated traditional Chinese and Western Medicine, Binzhou Medical College, Yantai 264000, China
Life Science School, Yantai University, Yantai 264000, China
These authors contributed equally to this work.
* Authors to whom correspondence should be addressed.
Received: 3 May 2013; in revised form: 16 July 2013 / Accepted: 22 July 2013 / Published: 24 July 2013
Abstract: The aim of this study was to evaluate the antitumour activities and genotoxic effects of isoliquiritigenin (ISL) combined with cyclophosphamide (CP) in vitro and in vivo. U14 cells were treated with either of ISL (5–25 μg/mL) or CP (0.25–1.25 mg/mL) alone or with combination of ISL (5–25 μg/mL) and CP (1.0 mg/mL) for 48 h. The proliferation inhibitory effect in vitro was evaluated by MTT and colony formation assays. KM mice bearing U14 mouse cervical cancer cells were used to estimate the antitumour activity in vivo. The genotoxic activity in bone marrow polychromatic erythrocytes was assayed by frequency of micronuclei. The DNA damage in peripheral white blood cells was assayed by single cell gel electrophoresis. The results showed that ISL enhanced antitumour activity of CP in vitro and in vivo, and decreased the micronucleus formation in polychromatic erythrocytes and DNA strand breaks in white blood cells in a dose-dependent way.
Keywords: isoliquiritigenin; antitumour; cyclophosphamide; genotoxic effect
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Zhao, H.; Yuan, X.; Li, D.; Chen, H.; Jiang, J.; Wang, Z.; Sun, X.; Zheng, Q. Isoliquiritigen Enhances the Antitumour Activity and Decreases the Genotoxic Effect of Cyclophosphamide. Molecules 2013, 18, 8786-8798.
Zhao H, Yuan X, Li D, Chen H, Jiang J, Wang Z, Sun X, Zheng Q. Isoliquiritigen Enhances the Antitumour Activity and Decreases the Genotoxic Effect of Cyclophosphamide. Molecules. 2013; 18(8):8786-8798.
Zhao, Hong; Yuan, Xuan; Li, Defang; Chen, Hongmei; Jiang, Jiangtao; Wang, Zhiping; Sun, Xiling; Zheng, Qiusheng. 2013. "Isoliquiritigen Enhances the Antitumour Activity and Decreases the Genotoxic Effect of Cyclophosphamide." Molecules 18, no. 8: 8786-8798.