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Molecules 2013, 18(8), 8725-8739; doi:10.3390/molecules18088725
Article

Polyphenolic Composition, Antioxidant and Antibacterial Activities for Two Romanian Subspecies of Achillea distans Waldst. et Kit. ex Willd.

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1 Department of Pharmacognosy "Iuliu Hatieganu" University of Medicine and Pharmacy, 12 I. Creanga Street, Cluj-Napoca 400010, Romania 2 Department of Pharmaceutical Technology and Biopharmaceutics, 12 I. Creanga Street, Cluj-Napoca 400010, Romania 3 Department of Chemistry and Chemical Engineering "Babes-Bolyai"University, 11 A. Janos Street, Cluj-Napoca 400028, Romania 4 Department of Physics, "Babes-Bolyai"University, 11 A. Janos Street, Cluj-Napoca 400028, Romania 5 State Veterinary Laboratory for Animal Health and Safety, 1 Piata Marasti Street, Cluj-Napoca 400609, Romania
* Author to whom correspondence should be addressed.
Received: 1 July 2013 / Revised: 18 July 2013 / Accepted: 18 July 2013 / Published: 24 July 2013
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Abstract

The aim of this work was to study the chemical composition, antioxidant and antibacterial properties of Achillea distans Waldst. et Kit. subsp. distans and Achillea distans Waldst. et Kit. subsp. alpina Rochel, from the Rodna Mountains (Romania). The identification and quantification of major phenolic compounds was performed by a HPLC-MS method. The total polyphenolic and flavonoid content was determined spectrophotometrically. The antioxidant activity was evaluated using the DPPH bleaching method, trolox equivalent antioxidant capacity assay (TEAC), hemoglobin ascorbate peroxidase activity inhibition (HAPX) assay, and an Electron Paramagnetic Resonance (EPR) spectroscopy method. A data indicated that A. distans subsp. alpina extract has more antioxidant activity than A. distans subsp. distans extract. Luteolin, apigenin, quercetin, caffeic and chlorogenic acids were present in the two extracts of A. distans, but in different amounts. Three flavonoids were detected only in A. distans subsp. alpina. The polyphenol-richer A. distans subsp. alpina extract showed a higher antioxidant activity than A. distans subsp. distans extract. A. distans subsp. distans extract showed inhibitory activity for Gram-positive bacteria, as evaluated with four species. The quantitative and qualitative differences between the two subspecies of Achillea distans could be used as a potential taxonomic marker in order to distinguish the species.
Keywords: Achillea distans; polyphenols; antioxidant activity; hemoglobin; EPR Achillea distans; polyphenols; antioxidant activity; hemoglobin; EPR
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Benedec, D.; Vlase, L.; Oniga, I.; Mot, A.C.; Damian, G.; Hanganu, D.; Duma, M.; Silaghi-Dumitrescu, R. Polyphenolic Composition, Antioxidant and Antibacterial Activities for Two Romanian Subspecies of Achillea distans Waldst. et Kit. ex Willd.. Molecules 2013, 18, 8725-8739.

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