Effect of Leguminous Lectins on the Growth of  Rhizobium tropici CIAT899

doi:10.3390/molecules18055792

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Molecules 2013, 18(5), 5792-5803; doi:10.3390/molecules18055792

Article

Effect of Leguminous Lectins on the Growth of Rhizobium tropici CIAT899

Mayron Alves de Vasconcelos¹, Cláudio Oliveira Cunha¹, Francisco Vassiliepe Sousa Arruda², Victor Alves Carneiro¹, Rafaela Mesquita Bastos², Fábio Martins Mercante^3,†, Kyria Santiago do Nascimento^1,†, Benildo Sousa Cavada^1,†, Ricardo Pires dos Santos^4,† and Edson Holanda Teixeira^2,†,*

¹ Department of Biochemistry and Molecular Biology, Federal University of Ceará, Fortaleza, CE, 60440-970, Brazil ² Integrated Laboratory of Biomolecules (LIBS), Department of Pathology and Legal Medicine, Faculty of Medicine, Federal University of Ceará, Fortaleza, CE, 60430-160, Brazil ³ Embrapa Western Region Agriculture, Dourados, MS, 79804-970, Brazil ⁴ Laboratory of Materials Engineering and Computation of Sobral (LEMCS), Federal University of Ceará, Campus do Derby, Sobral, CE, 62042-280, Brazil ^† Senior investigators of CNPq (Brazil).

* Author to whom correspondence should be addressed.

Received: 8 March 2013; Accepted: 14 May 2013 / Published: 17 May 2013

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Abstract: Rhizobium tropici is a Gram-negative bacterium that induces nodules and fixed atmospheric nitrogen in symbiotic association with Phaseolus vulgaris (common bean) and some other leguminous species. Lectins are proteins that specifically bind to carbohydrates and, consequently, modulate different biological functions. In this study, the d-glucose/ d-mannose-binding lectins (from seeds of Dioclea megacarpa, D. rostrata and D. violacea) and D-galactose-binding lectins (from seeds of Bauhinia variegata, Erythina velutina and Vatairea macrocarpa) were purified using chromatographic techniques and evaluated for their effect on the growth of R. tropici CIAT899. All lectins were assayed with a satisfactory degree of purity according to SDS-PAGE analysis, and stimulated bacterial growth; in particular, the Dioclea rostrata lectin was the most active among all tested proteins. As confirmed in the present study, both d-galactose- and d-glucose/d-mannose-binding lectins purified from the seeds of leguminous plants may be powerful biotechnological tools to stimulate the growth of R. tropici CIAT99, thus improving symbiotic interaction between rhizobia and common bean and, hence, the production of this field crop.

Keywords: rhizobia; Phaseolus vulgaris; stimulus; inoculant additive

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Cite This Article

MDPI and ACS Style

de Vasconcelos, M.A.; Cunha, C.O.; Arruda, F.V.S.; Carneiro, V.A.; Bastos, R.M.; Mercante, F.M.; Nascimento, K.S.; Cavada, B.S.; Santos, R.P.; Teixeira, E.H. Effect of Leguminous Lectins on the Growth of Rhizobium tropici CIAT899. Molecules 2013, 18, 5792-5803.

AMA Style

de Vasconcelos MA, Cunha CO, Arruda FVS, Carneiro VA, Bastos RM, Mercante FM, Nascimento KS, Cavada BS, Santos RP, Teixeira EH. Effect of Leguminous Lectins on the Growth of Rhizobium tropici CIAT899. Molecules. 2013; 18(5):5792-5803.

Chicago/Turabian Style

de Vasconcelos, Mayron A.; Cunha, Cláudio O.; Arruda, Francisco V.S.; Carneiro, Victor A.; Bastos, Rafaela M.; Mercante, Fábio M.; Nascimento, Kyria S.; Cavada, Benildo S.; Santos, Ricardo P.; Teixeira, Edson H. 2013. "Effect of Leguminous Lectins on the Growth of Rhizobium tropici CIAT899." Molecules 18, no. 5: 5792-5803.

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