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Isolation of Nematicidal Triterpenoid Saponins from Pulsatilla koreana Root and Their Activities against Meloidogyne incognita
College of Pharmacy, Chungnam National University, Daejeon 305-764, Korea
Technical Research Institute, Dongbang Agro Co., LTD, Buyeo, Chungnam 323-932, Korea
* Author to whom correspondence should be addressed.
Received: 18 April 2013; in revised form: 6 May 2013 / Accepted: 7 May 2013 / Published: 8 May 2013
Abstract: Pulsatilla koreana, a species endemic to Korea, is an important herb used in traditional medicine to treat amoebic dysentery and malaria. In the present study, 23 oleanane-type triterpenoid saponins 1–23 and eight lupane-type triterpenoid saponins 24–31 were isolated from the roots of P. koreana. Their structures were elucidated on the basis of spectroscopic data. The methanol extract and isolated compounds were next assessed for nematicidal activity against the root-knot nematode (Meloidogyne incognita). The methanol extract showed strong nematicidal activity after 48 h, with a LC50 value of 92.8 μg/mL. Compounds 2, 5, 9, 20, and 21 showed significant effects, with LC50 values ranging from 70.1 to 94.7 μg/mL after 48 h. These results suggest that triterpenoid saponins from P. koreana should be explored as potential natural nematicides for developing new agents to control root-knot nematode disease
Keywords: Pulsatilla koreana; triterpene saponin; Meloidogyne incognita; nematicidal activity
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Li, W.; Sun, Y.N.; Yan, X.T.; Yang, S.Y.; Lee, S.J.; Byun, H.J.; Moon, C.S.; Han, B.S.; Kim, Y.H. Isolation of Nematicidal Triterpenoid Saponins from Pulsatilla koreana Root and Their Activities against Meloidogyne incognita. Molecules 2013, 18, 5306-5316.
Li W, Sun YN, Yan XT, Yang SY, Lee SJ, Byun HJ, Moon CS, Han BS, Kim YH. Isolation of Nematicidal Triterpenoid Saponins from Pulsatilla koreana Root and Their Activities against Meloidogyne incognita. Molecules. 2013; 18(5):5306-5316.
Li, Wei; Sun, Ya N.; Yan, Xi T.; Yang, Seo Y.; Lee, Suk J.; Byun, Hyo J.; Moon, Chang S.; Han, Byung S.; Kim, Young H. 2013. "Isolation of Nematicidal Triterpenoid Saponins from Pulsatilla koreana Root and Their Activities against Meloidogyne incognita." Molecules 18, no. 5: 5306-5316.