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Simultaneous Determination of Structurally Diverse Compounds in Different Fangchi Species by UHPLC-DAD and UHPLC-ESI-MS/MS
College of Pharmacy, Kyung Hee University, Hoegi-dong, Dongdaemoon-gu, Seoul 130-701, Korea
Division of Metrology for Quality of Life, Korea Research Institute of Standards and Science, Yuseong, Daejeon 305-600, Korea
College of Pharmacy, Sungkyunkwan University, 300 Chonchon-dong, Jangan-ku, Suwon 440-746, Korea
These authors contributed equally to this work.
* Author to whom correspondence should be addressed.
Received: 25 March 2013; in revised form: 2 May 2013 / Accepted: 3 May 2013 / Published: 7 May 2013
Abstract: Two bisbenzylisoquinoline alkaloids, two morphine alkaloids, one aporphine alkaloid, syringaresinol and aristolochic acid І were selected as marker compounds and simultaneously analyzed using an ultra-high pressure liquid chromatography-diode array detection (UHPLC-DAD) method. These marker compounds were used for the quality control of Fangchi species of different origins, including Sinomenium acutum, Stephania tetrandra, Cocculus trilobus and Aristolochia fangchi. A reversed-phase UHPLC-DAD method was developed and validated for the simultaneous quantification of structurally diverse markers in different Fangchi species. In addition, an UHPLC-electrospray ionization tandem mass spectrometry (ESI-MS/MS) method was used for marker identification in Fangchi species, which provided diagnostic MS/MS spectral patterns that were dependent upon the marker structures. The UHPLC-MS/MS data were used to confirm and complement the UHPLC-DAD quality evaluation results. Additionally, magnoflorine and syringaresinol were observed for the first time in S. tetrandra and C. trilobus, respectively. Twenty different Fangchi species samples were analyzed for aristolochic acid I, syringaresinol and the alkaloids using the UHPLC-DAD and MS/MS method. Based on the levels of markers and principal component analysis (PCA), this method allowed for the clear classification of the samples into four different groups representing samples originating from the four species.
Keywords: Fangchi species; alkaloids; aristolochic acid I; lignan; UHPLC-DAD; UHPLC-MS/MS
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Sim, H.-J.; Kim, J.H.; Lee, K.R.; Hong, J. Simultaneous Determination of Structurally Diverse Compounds in Different Fangchi Species by UHPLC-DAD and UHPLC-ESI-MS/MS. Molecules 2013, 18, 5235-5250.
Sim H-J, Kim JH, Lee KR, Hong J. Simultaneous Determination of Structurally Diverse Compounds in Different Fangchi Species by UHPLC-DAD and UHPLC-ESI-MS/MS. Molecules. 2013; 18(5):5235-5250.
Sim, Hee-Jung; Kim, Ji H.; Lee, Kang R.; Hong, Jongki. 2013. "Simultaneous Determination of Structurally Diverse Compounds in Different Fangchi Species by UHPLC-DAD and UHPLC-ESI-MS/MS." Molecules 18, no. 5: 5235-5250.