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Molecules 2013, 18(5), 5235-5250; doi:10.3390/molecules18055235
Article

Simultaneous Determination of Structurally Diverse Compounds in Different Fangchi Species by UHPLC-DAD and UHPLC-ESI-MS/MS

1,2,†
, 1,†
, 3
 and 1,*
Received: 25 March 2013; in revised form: 2 May 2013 / Accepted: 3 May 2013 / Published: 7 May 2013
(This article belongs to the Section Metabolites)
Download PDF [440 KB, uploaded 18 June 2014]
Abstract: Two bisbenzylisoquinoline alkaloids, two morphine alkaloids, one aporphine alkaloid, syringaresinol and aristolochic acid І were selected as marker compounds and simultaneously analyzed using an ultra-high pressure liquid chromatography-diode array detection (UHPLC-DAD) method. These marker compounds were used for the quality control of Fangchi species of different origins, including Sinomenium acutum, Stephania tetrandra, Cocculus trilobus and Aristolochia fangchi. A reversed-phase UHPLC-DAD method was developed and validated for the simultaneous quantification of structurally diverse markers in different Fangchi species. In addition, an UHPLC-electrospray ionization tandem mass spectrometry (ESI-MS/MS) method was used for marker identification in Fangchi species, which provided diagnostic MS/MS spectral patterns that were dependent upon the marker structures. The UHPLC-MS/MS data were used to confirm and complement the UHPLC-DAD quality evaluation results. Additionally, magnoflorine and syringaresinol were observed for the first time in S. tetrandra and C. trilobus, respectively. Twenty different Fangchi species samples were analyzed for aristolochic acid I, syringaresinol and the alkaloids using the UHPLC-DAD and MS/MS method. Based on the levels of markers and principal component analysis (PCA), this method allowed for the clear classification of the samples into four different groups representing samples originating from the four species.
Keywords: Fangchi species; alkaloids; aristolochic acid I; lignan; UHPLC-DAD; UHPLC-MS/MS Fangchi species; alkaloids; aristolochic acid I; lignan; UHPLC-DAD; UHPLC-MS/MS
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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MDPI and ACS Style

Sim, H.-J.; Kim, J.H.; Lee, K.R.; Hong, J. Simultaneous Determination of Structurally Diverse Compounds in Different Fangchi Species by UHPLC-DAD and UHPLC-ESI-MS/MS. Molecules 2013, 18, 5235-5250.

AMA Style

Sim H-J, Kim JH, Lee KR, Hong J. Simultaneous Determination of Structurally Diverse Compounds in Different Fangchi Species by UHPLC-DAD and UHPLC-ESI-MS/MS. Molecules. 2013; 18(5):5235-5250.

Chicago/Turabian Style

Sim, Hee-Jung; Kim, Ji H.; Lee, Kang R.; Hong, Jongki. 2013. "Simultaneous Determination of Structurally Diverse Compounds in Different Fangchi Species by UHPLC-DAD and UHPLC-ESI-MS/MS." Molecules 18, no. 5: 5235-5250.


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