α-Glucosidase Inhibitory Effect and Simultaneous Quantification of Three Major Flavonoid Glycosides in Microctis folium
Abstract
:1. Introduction
2. Results and Discussion
2.1. Separation and Structural Elucidation
2.3. Optimization of HPLC Condition
2.4. Optimization of Sample Extraction for HPLC Analysis
2.5. Method Validation
Flavonoid glycosides | RT (min) | Calibration curve a | R2 | Test range (µg/mL) | LOD b (ng) | LOQ c (ng) |
---|---|---|---|---|---|---|
Vitexin (1) | 19.8 | y = 27.707x + 29.277 | 0.9999 | 3.2–206.0 | 0.6 | 1.9 |
Isovitexin (2) | 23.7 | y = 21.677x + 20.783 | 0.9999 | 3.3–214.0 | 0.7 | 2.5 |
Isorhamnetin 3-O-β-d-rutinoside (3) | 34.7 | y = 12.942x + 14.820 | 0.9999 | 3.3–212.0 | 0.5 | 1.5 |
Original (mg) | Spiked (mg) | Found (mg) | Recovery a (%) | R.S.D (%) | |
---|---|---|---|---|---|
Vitexin (1) | 0.55 | 1.03 | 103.6 | 1.98 | |
0.46 | 0.50 | 0.94 | 96.0 | 1.19 | |
0.38 | 0.83 | 97.4 | 0.98 | ||
Isovitexin (2) | 1.35 | 2.51 | 102.2 | 1.09 | |
1.13 | 1.10 | 2.24 | 103.6 | 0.99 | |
0.85 | 1.96 | 97.6 | 1.27 | ||
Isorhamnetin 3-O-β-d-rutinoside (3) | 0.88 | 1.59 | 98.9 | 2.21 | |
0.72 | 0.74 | 1.44 | 97.3 | 1.00 | |
0.59 | 1.30 | 98.3 | 1.28 |
2.6. Quantitative Analysis of Three Flavonoid Glycosides
Vitexin | Isovitexin | Isorhamnetin 3-O-β-d-Rutinoside | Total contents of 3 flavonoid glycosides | |
---|---|---|---|---|
BZY1 | 0.34 ± 0.00 | 1.80 ± 0.03 | 3.15 ± 0.03 | 5.30 ± 0.05 |
BZY2 | 0.20 ± 0.01 | 1.48 ± 0.03 | 3.18 ± 0.08 | 4.87 ± 0.11 |
BZY3 | 0.10 ± 0.00 | 1.17 ± 0.01 | 5.16 ± 0.01 | 6.42 ± 0.02 |
BZY4 | 0.61 ± 0.02 | 2.13 ± 0.05 | 2.55 ± 0.07 | 5.29 ± 0.13 |
BZY5 | 0.53 ± 0.00 | 1.97 ± 0.02 | 2.40 ± 0.01 | 4.90 ± 0.03 |
BZY6 | 0.30 ± 0.00 | 1.96 ± 0.02 | 4.01 ± 0.05 | 6.27 ± 0.07 |
BZY7 | 0.29 ± 0.01 | 1.43 ± 0.04 | 2.69 ± 0.06 | 4.41 ± 0.11 |
BZY8 | 1.32 ± 0.01 | 3.30 ± 0.02 | 3.06 ± 0.03 | 7.68 ± 0.06 |
BZY9 | 2.31 ± 0.04 | 5.65 ± 0.08 | 3.60 ± 0.05 | 11.55 ± 0.17 |
BZY10 | 0.46 ± 0.02 | 2.43 ± 0.04 | 2.39 ± 0.09 | 5.28 ± 0.14 |
BZY11 | 0.85 ± 0.04 | 1.73 ± 0.06 | 1.66 ± 0.06 | 4.23 ± 0.16 |
3. Experimental
3.1. Chemicals
3.2. Plant Materials
3.3. Extraction and Isolation
3.4. Samples Preparation for α-Glucosidase Inhibitory Assay
3.5. Assay of α-Glucosidase Inhibitory Activity
3.6. Samples Preparation for HPLC Analysis
3.7. Chromatographic Conditions
3.8. Method Validation of HPLC Analysis
4. Conclusions
Acknowledgments
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Chen, Y.-G.; Li, P.; Li, P.; Yan, R.; Zhang, X.-Q.; Wang, Y.; Zhang, X.-T.; Ye, W.-C.; Zhang, Q.-W. α-Glucosidase Inhibitory Effect and Simultaneous Quantification of Three Major Flavonoid Glycosides in Microctis folium. Molecules 2013, 18, 4221-4232. https://doi.org/10.3390/molecules18044221
Chen Y-G, Li P, Li P, Yan R, Zhang X-Q, Wang Y, Zhang X-T, Ye W-C, Zhang Q-W. α-Glucosidase Inhibitory Effect and Simultaneous Quantification of Three Major Flavonoid Glycosides in Microctis folium. Molecules. 2013; 18(4):4221-4232. https://doi.org/10.3390/molecules18044221
Chicago/Turabian StyleChen, Yan-Gan, Ping Li, Peng Li, Ru Yan, Xiao-Qi Zhang, Ying Wang, Xian-Tao Zhang, Wen-Cai Ye, and Qing-Wen Zhang. 2013. "α-Glucosidase Inhibitory Effect and Simultaneous Quantification of Three Major Flavonoid Glycosides in Microctis folium" Molecules 18, no. 4: 4221-4232. https://doi.org/10.3390/molecules18044221