Next Article in Journal
Repeated Oral Administration of Oleanolic Acid Produces Cholestatic Liver Injury in Mice
Previous Article in Journal
Monoterpenoids from Acanthopanax sessiliflorus Fruits
Article Menu

Export Article

Open AccessArticle
Molecules 2013, 18(3), 3050-3059; doi:10.3390/molecules18033050

A UPLC-MS/MS Method for Qualification of Quercetin-3-O-β- D-glucopyranoside-(4®1)-α-L-rhamnoside in Rat Plasma and Application to Pharmacokinetic Studies

Jiangsu Key Laboratory for High Technology of TCM Formulae Research, Nanjing University of Chinese Medicine, Nanjing 210046, China
*
Author to whom correspondence should be addressed.
Received: 31 January 2013 / Revised: 22 February 2013 / Accepted: 26 February 2013 / Published: 7 March 2013
(This article belongs to the Section Natural Products)
View Full-Text   |   Download PDF [333 KB, uploaded 18 June 2014]   |  

Abstract

A sensitive and accurate ultra-performance liquid chromatography coupled with triple quadrupole mass (UPLC-MS/MS) method was developed for the determination of quercetin-3-O-β-D-glucopyranoside-(4→1)-α-L-rhamnoside (QGR) in rat plasma using rutin as internal standard. Chromatographic separation was achieved on a Acquity BEH C18 column (100 mm × 2.1 mm, 1.7 μm) with a gradient elution of acetonitrile and 0.10% formic acid (v/v) at a flow rate of 0.4 mL/min. QGR and rutin were detected using electrospray negative ionization mass spectrometry in the multiple reaction monitoring (MRM) mode. The method demonstrated good linearity and did not show any endogenous interference with the QGR and rutin peaks. This method was successfully applied to a pharmacokinetic study of QGR in rats after intravenous (20 mg/kg) and oral (40 mg/kg) administration, and the results showed that the compound was poorly absorbed, with an absolute bioavailability of approximately 3.41%. View Full-Text
Keywords: quercetin-3-O-β-D-glucopyranoside-(4→1)-α-L-rhamnoside; pharmacokinetics; UPLC-MS/MS quercetin-3-O-β-D-glucopyranoside-(4→1)-α-L-rhamnoside; pharmacokinetics; UPLC-MS/MS
This is an open access article distributed under the Creative Commons Attribution License (CC BY 3.0).

Scifeed alert for new publications

Never miss any articles matching your research from any publisher
  • Get alerts for new papers matching your research
  • Find out the new papers from selected authors
  • Updated daily for 49'000+ journals and 6000+ publishers
  • Define your Scifeed now

SciFeed Share & Cite This Article

MDPI and ACS Style

Yao, X.; Zhou, G.; Tang, Y.; Li, Z.; Su, S.; Qian, D.; Duan, J.-A. A UPLC-MS/MS Method for Qualification of Quercetin-3-O-β- D-glucopyranoside-(4®1)-α-L-rhamnoside in Rat Plasma and Application to Pharmacokinetic Studies. Molecules 2013, 18, 3050-3059.

Show more citation formats Show less citations formats

Related Articles

Article Metrics

Article Access Statistics

1

Comments

[Return to top]

Molecules EISSN 1420-3049 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert
Back to Top