Molecules 2013, 18(3), 3050-3059; doi:10.3390/molecules18033050
Article

A UPLC-MS/MS Method for Qualification of Quercetin-3-O-β- D-glucopyranoside-(4®1)-α-L-rhamnoside in Rat Plasma and Application to Pharmacokinetic Studies

Jiangsu Key Laboratory for High Technology of TCM Formulae Research, Nanjing University of Chinese Medicine, Nanjing 210046, China
* Author to whom correspondence should be addressed.
Received: 31 January 2013; in revised form: 22 February 2013 / Accepted: 26 February 2013 / Published: 7 March 2013
(This article belongs to the Section Natural Products)
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Abstract: A sensitive and accurate ultra-performance liquid chromatography coupled with triple quadrupole mass (UPLC-MS/MS) method was developed for the determination of quercetin-3-O-β-D-glucopyranoside-(4→1)-α-L-rhamnoside (QGR) in rat plasma using rutin as internal standard. Chromatographic separation was achieved on a Acquity BEH C18 column (100 mm × 2.1 mm, 1.7 μm) with a gradient elution of acetonitrile and 0.10% formic acid (v/v) at a flow rate of 0.4 mL/min. QGR and rutin were detected using electrospray negative ionization mass spectrometry in the multiple reaction monitoring (MRM) mode. The method demonstrated good linearity and did not show any endogenous interference with the QGR and rutin peaks. This method was successfully applied to a pharmacokinetic study of QGR in rats after intravenous (20 mg/kg) and oral (40 mg/kg) administration, and the results showed that the compound was poorly absorbed, with an absolute bioavailability of approximately 3.41%.
Keywords: quercetin-3-O-β-D-glucopyranoside-(4→1)-α-L-rhamnoside; pharmacokinetics; UPLC-MS/MS

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MDPI and ACS Style

Yao, X.; Zhou, G.; Tang, Y.; Li, Z.; Su, S.; Qian, D.; Duan, J.-A. A UPLC-MS/MS Method for Qualification of Quercetin-3-O-β- D-glucopyranoside-(4®1)-α-L-rhamnoside in Rat Plasma and Application to Pharmacokinetic Studies. Molecules 2013, 18, 3050-3059.

AMA Style

Yao X, Zhou G, Tang Y, Li Z, Su S, Qian D, Duan J-A. A UPLC-MS/MS Method for Qualification of Quercetin-3-O-β- D-glucopyranoside-(4®1)-α-L-rhamnoside in Rat Plasma and Application to Pharmacokinetic Studies. Molecules. 2013; 18(3):3050-3059.

Chicago/Turabian Style

Yao, Xin; Zhou, Guisheng; Tang, Yuping; Li, Zhenhao; Su, Shulan; Qian, Dawei; Duan, Jin-Ao. 2013. "A UPLC-MS/MS Method for Qualification of Quercetin-3-O-β- D-glucopyranoside-(4®1)-α-L-rhamnoside in Rat Plasma and Application to Pharmacokinetic Studies." Molecules 18, no. 3: 3050-3059.

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