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• Peng, B
• Qiao, C
• Zhao, J
• Huang, W
• Hu, D
• Liu, H
• Li, S
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• Peng, B
• Qiao, C
• Zhao, J
• Huang, W
• Hu, D
• Liu, H
• Li, S
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• Qiao, C
• Zhao, J
• Huang, W
• Hu, D
• Liu, H
• Li, S

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Molecules 2013, 18(3), 2934-2941; doi:10.3390/molecules18032934

Article

Simultaneous Determination of Flavonoids, Isochlorogenic Acids and Triterpenoids in Ilex hainanensis Using High Performance Liquid Chromatography Coupled with Diode Array and Evaporative Light Scattering Detection

Bo Peng ^1,† , Chun-Feng Qiao ^1,† , Jing Zhao ^1,† , Wei-Hua Huang ¹ , De-Jun Hu ¹ , Hua-Gang Liu ^2,*  and Shao-Ping Li ^1,*

¹ State Key Laboratory of Quality Research in Chinese Medicine, Institute of Chinese Medical Sciences, University of Macau, Macao SAR, China ² School of Pharmaceutical Sciences, Guangxi Medical University, Nanning 530021, China ^† These authors contribute equally to this work.

* Authors to whom correspondence should be addressed.

Received: 14 January 2013; in revised form: 7 February 2013 / Accepted: 27 February 2013 / Published: 4 March 2013

(This article belongs to the Special Issue Triterpenes and Triterpenoids)

Download PDF Full-Text [279 KB, uploaded 4 March 2013 10:52 CET]

Abstract: A high performance liquid chromatography coupled with diode array and evaporative light scattering detection (HPLC-DAD-ELSD) method for simultaneous determination of eight major bioactive compounds including two flavonoids (rutin and eriodictyol-7-O-β-D-glucopyranoside), two isochlorogenic acids (isochlorogenic acid A and isochlorogenic acid C) and four triterpenoids (ilexhainanoside D, ilexsaponin A₁, ilexgenin A and ursolic acid) in Ilex hainanensis has been developed for the first time. The 283 nm wavelength was chosen for determination of two flavonoids and two isochlorogenic acids. ELSD was applied to determine four triterpenoids. The analysis was performed on an Agilent Zorbax SB-C₁₈ column (250 × 4.6 mm i.d., 5 µm) with gradient elution of 0.2% formic acid in water and acetonitrile. The method was validated for linearity, limit of detection, limit of quantification, precision, repeatability and accuracy. The proposed method has been successfully applied for simultaneous quantification of the analytes in four samples of Ilex hainanensis, which is helpful for quality control of this plant.

Keywords: Ilex hainanensis; flavonoid; isochlorogenic acid; triterpenoid; HPLC-DAD-ELSD

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