Abstract: Metastasis is the major cause of cancer-related deaths. Targeting the process of metastasis has been proposed as a strategy to fight cancer. Scutellaria barbata D. Don (S. barbata), a traditional Chinese medicine, is used for treatment of many diseases, including cancer. This study aimed to determine the anti-metastatic effect of total flavonoids of S. barbata (TF-SB) using the human hepatocarcinoma MHCC97H cell line with high metastatic potential. Our results show that TF-SB could significantly inhibit the proliferation and invasion of MHCC97H cells in a dose-dependent manner. MMP-2 and MMP-9 expression were obviously decreased after TF-SB treatment at both the mRNA and protein level. TIMP-1 and TIMP-2 expression were simultaneously increased. The present study indicates that TF-SB could reduce the metastatic capability of MHCC97H cell, probably through decrease of the MMP expression, and simultaneous increase of the TIMP expression.
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Dai, Z.-J.; Wang, B.-F.; Lu, W.-F.; Wang, Z.-D.; Ma, X.-B.; Min, W.-L.; Kang, H.-F.; Wang, X.-J.; Wu, W.-Y. Total Flavonoids of Scutellaria barbata Inhibit Invasion of Hepatocarcinoma via MMP/TIMP in Vitro. Molecules 2013, 18, 934-950.
Dai Z-J, Wang B-F, Lu W-F, Wang Z-D, Ma X-B, Min W-L, Kang H-F, Wang X-J, Wu W-Y. Total Flavonoids of Scutellaria barbata Inhibit Invasion of Hepatocarcinoma via MMP/TIMP in Vitro. Molecules. 2013; 18(1):934-950.
Dai, Zhi-Jun; Wang, Bao-Feng; Lu, Wang-Feng; Wang, Zhi-Dong; Ma, Xiao-Bin; Min, Wei-Li; Kang, Hua-Feng; Wang, Xi-Jing; Wu, Wen-Ying. 2013. "Total Flavonoids of Scutellaria barbata Inhibit Invasion of Hepatocarcinoma via MMP/TIMP in Vitro." Molecules 18, no. 1: 934-950.