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Antitrypanosomal Alkaloids from the Marine Bacterium Bacillus pumilus
Center for Biodiversity and Drug Discovery, Institute for Scientific Research and High Technology Services, City of Knowledge, P.O. Box 0843-01103, Panama
Departamento de Química, Centro de Investigación y de Estudios Avanzados del IPN, México D.F. 07360, Mexico
Center for Cellular and Molecular Biology of Diseases, Institute for Scientific Research and High Technology Services, City of Knowledge, P.O. Box 0843-01103, Panama
Smithsonian Tropical Research Institute, Balboa, Ancon, P.O. Box 0843-03092, Panama
* Author to whom correspondence should be addressed.
Received: 18 July 2012; in revised form: 6 September 2012 / Accepted: 7 September 2012 / Published: 18 September 2012
Abstract: Fractionation of the ethyl acetate extract of the marine bacterium Bacillus pumilus isolated from the black coral Antipathes sp. led to the isolation of five compounds: cyclo-(L-Leu-L-Pro) (1), 3-hydroxyacetylindole (2), N-acetyl-b-oxotryptamine (3), cyclo-(L-Phe-L-Pro) (4), and 3-formylindole (5). The structures of compounds 1−5 were established by spectroscopic analyses, including HRESITOF-MS and NMR (1H, 13C, HSQC, HMBC and COSY). Compounds 2, 3 and 5 caused the inhibition on the growth of Trypanosoma cruzi (T. cruzi), with IC50 values of 20.6, 19.4 and 26.9 μM, respectively, with moderate cytotoxicity against Vero cells. Compounds 1−5 were found to be inactive when tested against Plasmodium falciparum and Leishmania donovani, therefore showing selectivity against T. cruzi parasites.
Keywords: indole alkaloids; Trypanosoma cruzi; Bacillus pumilus; Chagas disease
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Martínez-Luis, S.; Gómez, J.F.; Spadafora, C.; Guzmán, H.M.; Gutiérrez, M. Antitrypanosomal Alkaloids from the Marine Bacterium Bacillus pumilus. Molecules 2012, 17, 11146-11155.
Martínez-Luis S, Gómez JF, Spadafora C, Guzmán HM, Gutiérrez M. Antitrypanosomal Alkaloids from the Marine Bacterium Bacillus pumilus. Molecules. 2012; 17(9):11146-11155.
Martínez-Luis, Sergio; Gómez, José Félix; Spadafora, Carmenza; Guzmán, Héctor M.; Gutiérrez, Marcelino. 2012. "Antitrypanosomal Alkaloids from the Marine Bacterium Bacillus pumilus." Molecules 17, no. 9: 11146-11155.