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Molecules 2012, 17(9), 10831-10845; doi:10.3390/molecules170910831
Article

Protective Effects of the Key Compounds Isolated from Corni fructus against β-Amyloid-Induced Neurotoxicity in PC12 Cells

1,†
, 2,†
 and 1,*
Received: 16 July 2012; in revised form: 1 September 2012 / Accepted: 5 September 2012 / Published: 10 September 2012
(This article belongs to the collection Bioactive Compounds)
Download PDF [573 KB, updated 18 June 2014; original version uploaded 18 June 2014]
Abstract: β-Amyloid (Aβ) peptide is the major component of senile plaques and is considered to have a causal role in the development and progression of Alzheimer’s disease (AD). There is compelling evidence supporting the notion that Aβ-induced cytotoxicity is mediated though the generation of ROS. In the present study, we investigated the neuroprotective effects of ursolic acid (UA), p-coumaric acid (p-CA), and gallic acid (GA) isolated from Corni fructus (CF) against Aβ(25–35)-induced toxicity in PC12 cell. Exposure of PC12 cells to 50 μM Aβ(25–35) increased cellular oxidative stress, the number of apoptotic cells and caspase-3 activity and finally caused significant cell death. However, UA, p-CA, and GA not only suppressed the generation of ROS but also attenuated DNA fragmentation and eventually attenuated Aβ-induced apoptosis in a dose-dependent manner. In protecting cells against Aβ neurotoxicity, UA and GA possessed stronger ability against ROS generation than p-CA, while p-CA showed the strongest anti-apoptotic activity. Particularly, p-CA protected cells at the concentration range from 0.5 up to 125 μM without any adverse effect. Taken together, these effects of UA, p-CA, and GA may be partly associated with the neuroprotective effect of CF. Furthermore, our findings might raise a possibility of therapeutic applications of CF for preventing and/or treating neurodegenerative diseases.
Keywords: Alzheimer’s disease (AD); amyloid β peptide (Aβ); Corni fructus; apoptosis; anti-oxidant; neuroprotection Alzheimer’s disease (AD); amyloid β peptide (Aβ); Corni fructus; apoptosis; anti-oxidant; neuroprotection
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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MDPI and ACS Style

Hong, S.-Y.; Jeong, W.-S.; Jun, M. Protective Effects of the Key Compounds Isolated from Corni fructus against β-Amyloid-Induced Neurotoxicity in PC12 Cells. Molecules 2012, 17, 10831-10845.

AMA Style

Hong S-Y, Jeong W-S, Jun M. Protective Effects of the Key Compounds Isolated from Corni fructus against β-Amyloid-Induced Neurotoxicity in PC12 Cells. Molecules. 2012; 17(9):10831-10845.

Chicago/Turabian Style

Hong, Seung-Young; Jeong, Woo-Sik; Jun, Mira. 2012. "Protective Effects of the Key Compounds Isolated from Corni fructus against β-Amyloid-Induced Neurotoxicity in PC12 Cells." Molecules 17, no. 9: 10831-10845.


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