Preservation of Bacillus firmus Strain 37 and Optimization of Cyclodextrin Biosynthesis by Cells Immobilized on Loofa Sponge

doi:10.3390/molecules17089476

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Molecules 2012, 17(8), 9476-9488; doi:10.3390/molecules17089476

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Preservation of Bacillus firmus Strain 37 and Optimization of Cyclodextrin Biosynthesis by Cells Immobilized on Loofa Sponge

Rúbia Pazzetto¹, Sabrina Barbosa de Souza Ferreira¹, Elder James Silva Santos¹, Cristiane Moriwaki¹, Teresinha Aparecida Guedes² and Graciette Matioli^1,*

¹ Department of Pharmacy, State University of Maringá (UEM), Av. Colombo, 5790, Maringá-PR 87020-900, Brazil ² Department of Statistics, State University of Maringá (UEM), Av. Colombo, 5790, Maringá-PR 87020-900, Brazil

* Author to whom correspondence should be addressed.

Received: 28 April 2012; in revised form: 18 July 2012 / Accepted: 21 July 2012 / Published: 8 August 2012

(This article belongs to the Section Natural Products)

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Abstract: The preservation of Bacillus firmus strain 37 cells by lyophilization was evaluated and response surface methodology (RSM) was used to optimize the β-cyclodextrin (β-CD) production by cells immobilized on loofa sponge. Interactions were studied with the variables temperature, pH and dextrin concentration using a central composite design (CCD). Immobilization time influence on β-CD production was also investigated. B. firmus strain 37 cells remained viable after one year of storage, showing that the lyophilization is a suitable method for preservation of the microorganism. From the three-dimensional diagrams and contour plots, the best conditions for β-CD production were determined: temperature 60 °C, pH 8, and 18% dextrin. Considering that the amount of dextrin was high, a new assay was carried out, in which dextrin concentrations of 10, 15, and 18% were tested and the temperature of 60 °C and pH 8 were maintained. The results achieved showed very small differences and therefore, for economic reasons, the use of 10% dextrin is suggested. Increasing the immobilization time of cells immobilized on synthetic sponge the β-CD production decreased and did not change for cells immobilized on loofa sponge. The results of this research are important for microorganism preservation and essential in the optimization of the biosynthesis of CD.

Keywords: microorganism preservation; response surface methodology; cyclodextrin; loofa sponge; cells immobilization; Bacillus firmus

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MDPI and ACS Style

Pazzetto, R.; Ferreira, S.B.S.; Santos, E.J.S.; Moriwaki, C.; Guedes, T.A.; Matioli, G. Preservation of Bacillus firmus Strain 37 and Optimization of Cyclodextrin Biosynthesis by Cells Immobilized on Loofa Sponge. Molecules 2012, 17, 9476-9488.

AMA Style

Pazzetto R, Ferreira SBS, Santos EJS, Moriwaki C, Guedes TA, Matioli G. Preservation of Bacillus firmus Strain 37 and Optimization of Cyclodextrin Biosynthesis by Cells Immobilized on Loofa Sponge. Molecules. 2012; 17(8):9476-9488.

Chicago/Turabian Style

Pazzetto, Rúbia; Ferreira, Sabrina Barbosa de Souza; Santos, Elder James Silva; Moriwaki, Cristiane; Guedes, Teresinha Aparecida; Matioli, Graciette. 2012. "Preservation of Bacillus firmus Strain 37 and Optimization of Cyclodextrin Biosynthesis by Cells Immobilized on Loofa Sponge." Molecules 17, no. 8: 9476-9488.

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