Next Article in Journal
Chiral Zn(II)-Bisamidine Complex as a Lewis-Brønsted Combined Acid Catalyst: Application to Asymmetric Mukaiyama Aldol Reactions of α-Ketoesters
Previous Article in Journal
Prediction of Acute Mammalian Toxicity Using QSAR Methods: A Case Study of Sulfur Mustard and Its Breakdown Products
Molecules 2012, 17(8), 9002-9009; doi:10.3390/molecules17089002
Article

Preparative Isolation and Purification of Three Sesquiterpenoid Lactones from Eupatorium lindleyanum DC. by High-Speed Counter-Current Chromatography

1,2,3, 2,3, 2 and 1,*
1 College of Biotechnology and Pharmaceutical Engineering, Nanjing University of Technology, Nanjing 210009, China 2 Jiangsu Key Laboratory for Eco-Agricultural Biotechnology around Hongze Lake, Huaiyin Normal University, Huaian 223300, China 3 Jiangsu Key Laboratory for Biomass-based Energy and Enzyme Technology, Huaiyin Normal University, Huaian 223300, China
* Author to whom correspondence should be addressed.
Received: 20 June 2012 / Revised: 18 July 2012 / Accepted: 21 July 2012 / Published: 27 July 2012
View Full-Text   |   Download PDF [270 KB, uploaded 18 June 2014]   |   Browse Figures
SciFeed

Abstract

A high-speed counter-current chromatography (HSCCC) method was established for the preparative separation of three sesquiterpenoid lactones from Eupatorium lindleyanum DC. The two-phase solvent system composed of n-hexane–ethyl acetate–methanol–water (1:4:2:3, v/v/v/v) was selected. From 540 mg of the n-butanol fraction of Eupatorium lindleyanum DC., 10.8 mg of 3β-hydroxy-8β-[4'-hydroxy-tigloyloxy]-costunolide, 17.9 mg of eupalinolide A and 19.3 mg of eupalinolide B were obtained in a one-step HSCCC separation, with purities of 91.8%, 97.9% and 97.1%, respectively, as determined by HPLC. Their structures were further identified by ESI-MS and 1H-NMR.
Keywords: Eupatorium lindleyanum DC.; high-speed counter-current chromatography; sesquiterpenoid lactone; separation Eupatorium lindleyanum DC.; high-speed counter-current chromatography; sesquiterpenoid lactone; separation
This is an open access article distributed under the Creative Commons Attribution License (CC BY 3.0).

Supplementary material

Share & Cite This Article

Further Mendeley | CiteULike
Export to BibTeX |
EndNote |
RIS
MDPI and ACS Style

Yan, G.; Ji, L.; Luo, Y.; Hu, Y. Preparative Isolation and Purification of Three Sesquiterpenoid Lactones from Eupatorium lindleyanum DC. by High-Speed Counter-Current Chromatography. Molecules 2012, 17, 9002-9009.

View more citation formats

Related Articles

Article Metrics

For more information on the journal, click here

Comments

[Return to top]
Molecules EISSN 1420-3049 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert