Molecules 2012, 17(8), 9002-9009; doi:10.3390/molecules17089002
Article

Preparative Isolation and Purification of Three Sesquiterpenoid Lactones from Eupatorium lindleyanum DC. by High-Speed Counter-Current Chromatography

Guilong Yan 1,2,3 , Lilian Ji 2,3 , Yuming Luo 2  and Yonghong Hu 1,* email
1 College of Biotechnology and Pharmaceutical Engineering, Nanjing University of Technology, Nanjing 210009, China 2 Jiangsu Key Laboratory for Eco-Agricultural Biotechnology around Hongze Lake, Huaiyin Normal University, Huaian 223300, China 3 Jiangsu Key Laboratory for Biomass-based Energy and Enzyme Technology, Huaiyin Normal University, Huaian 223300, China
* Author to whom correspondence should be addressed.
Received: 20 June 2012; in revised form: 18 July 2012 / Accepted: 21 July 2012 / Published: 27 July 2012
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Abstract: A high-speed counter-current chromatography (HSCCC) method was established for the preparative separation of three sesquiterpenoid lactones from Eupatorium lindleyanum DC. The two-phase solvent system composed of n-hexane–ethyl acetate–methanol–water (1:4:2:3, v/v/v/v) was selected. From 540 mg of the n-butanol fraction of Eupatorium lindleyanum DC., 10.8 mg of 3β-hydroxy-8β-[4'-hydroxy-tigloyloxy]-costunolide, 17.9 mg of eupalinolide A and 19.3 mg of eupalinolide B were obtained in a one-step HSCCC separation, with purities of 91.8%, 97.9% and 97.1%, respectively, as determined by HPLC. Their structures were further identified by ESI-MS and 1H-NMR.
Keywords: Eupatorium lindleyanum DC.; high-speed counter-current chromatography; sesquiterpenoid lactone; separation

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MDPI and ACS Style

Yan, G.; Ji, L.; Luo, Y.; Hu, Y. Preparative Isolation and Purification of Three Sesquiterpenoid Lactones from Eupatorium lindleyanum DC. by High-Speed Counter-Current Chromatography. Molecules 2012, 17, 9002-9009.

AMA Style

Yan G, Ji L, Luo Y, Hu Y. Preparative Isolation and Purification of Three Sesquiterpenoid Lactones from Eupatorium lindleyanum DC. by High-Speed Counter-Current Chromatography. Molecules. 2012; 17(8):9002-9009.

Chicago/Turabian Style

Yan, Guilong; Ji, Lilian; Luo, Yuming; Hu, Yonghong. 2012. "Preparative Isolation and Purification of Three Sesquiterpenoid Lactones from Eupatorium lindleyanum DC. by High-Speed Counter-Current Chromatography." Molecules 17, no. 8: 9002-9009.

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