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Molecules 2012, 17(8), 9002-9009; doi:10.3390/molecules17089002
Article

Preparative Isolation and Purification of Three Sesquiterpenoid Lactones from Eupatorium lindleyanum DC. by High-Speed Counter-Current Chromatography

1,2,3, 2,3, 2 and 1,*
Received: 20 June 2012 / Revised: 18 July 2012 / Accepted: 21 July 2012 / Published: 27 July 2012
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Abstract

A high-speed counter-current chromatography (HSCCC) method was established for the preparative separation of three sesquiterpenoid lactones from Eupatorium lindleyanum DC. The two-phase solvent system composed of n-hexane–ethyl acetate–methanol–water (1:4:2:3, v/v/v/v) was selected. From 540 mg of the n-butanol fraction of Eupatorium lindleyanum DC., 10.8 mg of 3β-hydroxy-8β-[4'-hydroxy-tigloyloxy]-costunolide, 17.9 mg of eupalinolide A and 19.3 mg of eupalinolide B were obtained in a one-step HSCCC separation, with purities of 91.8%, 97.9% and 97.1%, respectively, as determined by HPLC. Their structures were further identified by ESI-MS and 1H-NMR.
Keywords: Eupatorium lindleyanum DC.; high-speed counter-current chromatography; sesquiterpenoid lactone; separation Eupatorium lindleyanum DC.; high-speed counter-current chromatography; sesquiterpenoid lactone; separation
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Yan, G.; Ji, L.; Luo, Y.; Hu, Y. Preparative Isolation and Purification of Three Sesquiterpenoid Lactones from Eupatorium lindleyanum DC. by High-Speed Counter-Current Chromatography. Molecules 2012, 17, 9002-9009.

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