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A Sandwich HIV p24 Amperometric Immunosensor Based on a Direct Gold Electroplating-Modified Electrode
Clinical Laboratory Center, Nanfang Hospital, Southern Medical University, Guangzhou 510515, China
The State Key Laboratory Base of Novel Functional Materials and Preparation Science, Faculty of Material Science and Chemical Engineering of Ningbo University, Ningbo 315211, China
These authors contributed equally to this work.
* Authors to whom correspondence should be addressed.
Received: 8 March 2012; in revised form: 25 April 2012 / Accepted: 9 May 2012 / Published: 18 May 2012
Abstract: Acquired immune deficiency syndrome (AIDS) is a severe communicable immune deficiency disease caused by the human immune deficiency virus (HIV). The analysis laboratory diagnosis of HIV infection is a crucial aspect of controlling AIDS. The p24 antigen, the HIV-1 capsid protein, is of considerable diagnostic interest because it is detectable several days earlier than host-generated HIV antibodies following HIV exposure. We present herein a new sandwich HIV p24 immunosensor based on directly electroplating an electrode surface with gold nanoparticles using chronoamperometry, which greatly increased the conductivity and reversibility of the electrode. Under optimum conditions, the electrochemical signal showed a linear relationship with the concentration of p24, ranging from 0.01 ng/mL to 100 ng/mL (R > 0.99), and the detection limit was 0.008 ng/mL. Compared with ELISA, this method increased the sensitivity by more than two orders of magnitude (the sensitivity of ELISA for p24 is about 1 ng/mL). This immunosensor may be broadly applied to clinical samples, being distinguished by its ease of use, mild reaction conditions, guaranteed reproducibility, and good anti-interference ability.
Keywords: HIV; p24; sandwich amperometric immunosensor; direct electroplating
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MDPI and ACS Style
Zheng, L.; Jia, L.; Li, B.; Situ, B.; Liu, Q.; Wang, Q.; Gan, N. A Sandwich HIV p24 Amperometric Immunosensor Based on a Direct Gold Electroplating-Modified Electrode. Molecules 2012, 17, 5988-6000.
Zheng L, Jia L, Li B, Situ B, Liu Q, Wang Q, Gan N. A Sandwich HIV p24 Amperometric Immunosensor Based on a Direct Gold Electroplating-Modified Electrode. Molecules. 2012; 17(5):5988-6000.
Zheng, Lei; Jia, Liyong; Li, Bo; Situ, Bo; Liu, Qinlan; Wang, Qian; Gan, Ning. 2012. "A Sandwich HIV p24 Amperometric Immunosensor Based on a Direct Gold Electroplating-Modified Electrode." Molecules 17, no. 5: 5988-6000.