• Abstract
• Full-Text PDF [317 KB]

• Article Statistics
• PubMed/Medline
• Google Scholar
• Order Reprints

• Cite this article
• Export to BibTeX
• Export to EndNote

• [+] on DOAJ
• Boligon, A
• Sagrillo, M
• Machado, L
• de Souza Filho, O
• Machado, M
• da Cruz, I
• Athayde, M
• [+] on Google Scholar
• Boligon, A
• Sagrillo, M
• Machado, L
• de Souza Filho, O
• Machado, M
• da Cruz, I
• Athayde, M
• [+] on PubMed
• Boligon, A
• Sagrillo, M
• Machado, L
• de Souza Filho, O
• Machado, M
• da Cruz, I
• Athayde, M

•  CiteULike
•  Facebook
•  Mendeley
•  Twitter

This article is

• freely available
• re-usable

Molecules 2012, 17(5), 5757-5769; doi:10.3390/molecules17055757

Article

Protective Effects of Extracts and Flavonoids Isolated from Scutia buxifolia Reissek against Chromosome Damage in Human Lymphocytes Exposed to Hydrogen Peroxide

Aline Augusti Boligon ^1,* , Michele Rorato Sagrillo ^2,3 , Luiz Filipe Machado ³ , Olmiro de Souza Filho ³ , Michel Mansur Machado ⁴ , Ivana Beatrice Manica da Cruz ³  and Margareth Linde Athayde ¹

¹ Program of Post-Graduation in Pharmaceutical Sciences, Federal University of Santa Maria, Campus Camobi, Santa Maria, RS, 97105-900, Brazil ² Franciscan University Center – UNIFRA, Andradas Street, 1614, Santa Maria, RS 97010-032, Brazil ³ Program of Post-Graduation in Pharmacology, Federal University of Santa Maria, Campus Camobi, Santa Maria, RS, 97105-900, Brazil ⁴ Federal University of Pampa – UNIPAMPA, Uruguaiana, RS 97500-970, Brazil

* Author to whom correspondence should be addressed.

Received: 26 March 2012; in revised form: 25 April 2012 / Accepted: 26 April 2012 / Published: 14 May 2012

(This article belongs to the Section Natural Products)

Download PDF Full-Text [317 KB, uploaded 14 May 2012 11:47 CEST]

Abstract: Flavonoids are claimed to protect against cardiovascular disease, certain forms of cancer and ageing, possibly by preventing initial DNA damage. Therefore, we investigated the protective effects of crude extract, ethyl acetate fraction and flavonoids (quercetin, quercitrin, isoquercitrin and rutin) isolated from the leaves from Scutia buxifolia against chromosome damage induced by H₂O₂ in human lymphocytes by analyzing cellular growth rate, cell viability, mitotic index and chromosomal instability. We found a differential response among the compounds tested, with the ethyl acetate fraction being more effective than the crude extract, a difference perhaps related to the presence of the antioxidants identified and quantified by HPLC/DAD. In general, quercetin, isoquercitrin and rutin recovered the mitotic index and chromosomal instability more than quercitrin after treatment with hydrogen peroxide.

Keywords: Scutia buxifolia; flavonols; lymphocyte culture; chromosomes damage; HPLC/DAD

Article Statistics

Cite This Article