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Molecules 2012, 17(5), 5757-5769; doi:10.3390/molecules17055757
Article

Protective Effects of Extracts and Flavonoids Isolated from Scutia buxifolia Reissek against Chromosome Damage in Human Lymphocytes Exposed to Hydrogen Peroxide

1,* , 2,3, 3, 3, 4, 3 and 1
1 Program of Post-Graduation in Pharmaceutical Sciences, Federal University of Santa Maria, Campus Camobi, Santa Maria, RS, 97105-900, Brazil 2 Franciscan University Center – UNIFRA, Andradas Street, 1614, Santa Maria, RS 97010-032, Brazil 3 Program of Post-Graduation in Pharmacology, Federal University of Santa Maria, Campus Camobi, Santa Maria, RS, 97105-900, Brazil 4 Federal University of Pampa – UNIPAMPA, Uruguaiana, RS 97500-970, Brazil
* Author to whom correspondence should be addressed.
Received: 26 March 2012 / Revised: 25 April 2012 / Accepted: 26 April 2012 / Published: 14 May 2012
(This article belongs to the Section Metabolites)
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Abstract

Flavonoids are claimed to protect against cardiovascular disease, certain forms of cancer and ageing, possibly by preventing initial DNA damage. Therefore, we investigated the protective effects of crude extract, ethyl acetate fraction and flavonoids (quercetin, quercitrin, isoquercitrin and rutin) isolated from the leaves from Scutia buxifolia against chromosome damage induced by H2O2 in human lymphocytes by analyzing cellular growth rate, cell viability, mitotic index and chromosomal instability. We found a differential response among the compounds tested, with the ethyl acetate fraction being more effective than the crude extract, a difference perhaps related to the presence of the antioxidants identified and quantified by HPLC/DAD. In general, quercetin, isoquercitrin and rutin recovered the mitotic index and chromosomal instability more than quercitrin after treatment with hydrogen peroxide.
Keywords: Scutia buxifolia; flavonols; lymphocyte culture; chromosomes damage; HPLC/DAD Scutia buxifolia; flavonols; lymphocyte culture; chromosomes damage; HPLC/DAD
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Boligon, A.A.; Sagrillo, M.R.; Machado, L.F.; de Souza Filho, O.; Machado, M.M.; da Cruz, I.B.M.; Athayde, M.L. Protective Effects of Extracts and Flavonoids Isolated from Scutia buxifolia Reissek against Chromosome Damage in Human Lymphocytes Exposed to Hydrogen Peroxide. Molecules 2012, 17, 5757-5769.

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