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The Antimicrobial Efficacy of Elaeis guineensis: Characterization, in Vitro and in Vivo Studies
Institute for Research in Molecular Medicine (INFORMM), Universiti Sains Malaysia, Pulau Pinang 11800, Malaysia
Biology Program, School of Distance Education, Universiti Sains Malaysia, 11800 Minden, Penang, Malaysia
Dental Research & Training Unit, and Oral Cancer Research and Coordinating Centre (OCRCC), Faculty of Dentistry, University of Malaya, Kuala Lumpur 50603, Malaysia
Nanomedicine-Laboratory of Immunology and Molecular Biomedical Research (LIMBR), Centre for Biotechnology and Interdisciplinary Biosciences (BioDeakin), Institute for Frontier Materials (IFM), Deakin University, Waurn Ponds, Victoria 3217, Australia
* Author to whom correspondence should be addressed.
Received: 6 March 2012; in revised form: 14 April 2012 / Accepted: 16 April 2012 / Published: 26 April 2012
Abstract: The urgent need to treat multi-drug resistant pathogenic microorganisms in chronically infected patients has given rise to the development of new antimicrobials from natural resources. We have tested Elaeis guineensis Jacq (Arecaceae) methanol extract against a variety of bacterial, fungal and yeast strains associated with infections. Our studies have demonstrated that E. guineensis exhibits excellent antimicrobial activity in vitro and in vivo against the bacterial and fungal strains tested. A marked inhibitory effect of the E. guineensis extracts was observed against C. albicans whereby E. guineensis extract at ½, 1, or 2 times the MIC significantly inhibited C. albicans growth with a noticeable drop in optical density (OD) of the bacterial culture. This finding confirmed the anticandidal activity of the extract on C. albicans. Imaging using scanning (SEM) and transmission (TEM) electron microscopy was done to determine the major alterations in the microstructure of the extract-treated C. albicans. The main abnormalities noted via SEM and TEM studies were the alteration in morphology of the yeast cells. In vivo antimicrobial activity was studies in mice that had been inoculated with C. albicans and exhibited good anticandidal activity. The authors conclude that the extract may be used as a candidate for the development of anticandidal agent.
Keywords: antimicrobial activity; Candida albicans; Elaeis guineensis; scanning electron microscopy; transmission electron microscopy
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Vijayarathna, S.; Zakaria, Z.; Chen, Y.; Latha, L.Y.; Kanwar, J.R.; Sasidharan, S. The Antimicrobial Efficacy of Elaeis guineensis: Characterization, in Vitro and in Vivo Studies. Molecules 2012, 17, 4860-4877.
Vijayarathna S, Zakaria Z, Chen Y, Latha LY, Kanwar JR, Sasidharan S. The Antimicrobial Efficacy of Elaeis guineensis: Characterization, in Vitro and in Vivo Studies. Molecules. 2012; 17(5):4860-4877.
Vijayarathna, Soundararajan; Zakaria, Zuraini; Chen, Yeng; Latha, Lachimanan Yoga; Kanwar, Jagat R.; Sasidharan, Sreenivasan. 2012. "The Antimicrobial Efficacy of Elaeis guineensis: Characterization, in Vitro and in Vivo Studies." Molecules 17, no. 5: 4860-4877.