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• Grujic, N
• Lepojevic, Z
• Srdjenovic, B
• Vladic, J
• Sudji, J
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• Grujic, N
• Lepojevic, Z
• Srdjenovic, B
• Vladic, J
• Sudji, J
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• Lepojevic, Z
• Srdjenovic, B
• Vladic, J
• Sudji, J

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Molecules 2012, 17(3), 2518-2528; doi:10.3390/molecules17032518

Article

Effects of Different Extraction Methods and Conditions on the Phenolic Composition of Mate Tea Extracts

Nevena Grujic ^1,* , Zika Lepojevic ² , Branislava Srdjenovic ¹ , Jelena Vladic ¹  and Jan Sudji ³

¹ Department of Pharmacy, Medical Faculty, University of Novi Sad, Serbia ² Department of Pharmaceutical Engineering, Faculty of Technology, University of Novi Sad, Serbia ³ The Institute for the Health Protection of Workers Novi Sad, Serbia

* Author to whom correspondence should be addressed.

Received: 12 January 2012; in revised form: 24 February 2012 / Accepted: 27 February 2012 / Published: 2 March 2012

Download PDF Full-Text [217 KB, uploaded 2 March 2012 10:47 CET]

Abstract: A simple and rapid HPLC method for determination of chlorogenic acid (5-O-caffeoylquinic acid) in mate tea extracts was developed and validated. The chromatography used isocratic elution with a mobile phase of aqueous 1.5% acetic acid-methanol (85:15, v/v). The flow rate was 0.8 mL/min and detection by UV at 325 nm. The method showed good selectivity, accuracy, repeatability and robustness, with detection limit of 0.26 mg/L and recovery of 97.76%. The developed method was applied for the determination of chlorogenic acid in mate tea extracts obtained by ethanol extraction and liquid carbon dioxide extraction with ethanol as co-solvent. Different ethanol concentrations were used (40, 50 and 60%, v/v) and liquid CO₂ extraction was performed at different pressures (50 and 100 bar) and constant temperature (27 ± 1 °C). Significant influence of extraction methods, conditions and solvent polarity on chlorogenic acid content, antioxidant activity and total phenolic and flavonoid content of mate tea extracts was established. The most efficient extraction solvent was liquid CO₂ with aqueous ethanol (40%) as co-solvent using an extraction pressure of 100 bar.

Keywords: mate; chlorogenic acid; HPLC analysis; DPPH; total phenolic content; total flavonoid content

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