Molecules 2012, 17(3), 2335-2350; doi:10.3390/molecules17032335
Article

Comparative Studies of the (Anti) Mutagenicity of Baccharis dracunculifolia and Artepillin C by the Bacterial Reverse Mutation Test

1 Departamento de Ciências Biológicas, Faculdade de Ciências Farmacêuticas de Araraquara, Universidade Estadual Paulista Julio de Mesquita Filho,14801-902, Araraquara, São Paulo, Brazil 2 Universidade de Franca, 14404-600, Franca, São Paulo, Brazil 3 Faculdade de Ciências Farmacêuticas de Ribeirão Preto, Universidade de São Paulo, 14040-903, Ribeirão Preto, São Paulo, Brazil 4 Departamento de Ciências Farmacêuticas, Faculdade de Farmácia, Universidade Federal de Juiz de Fora, 36035-900, Juiz de Fora, Minas Gerais, Brazil
* Author to whom correspondence should be addressed.
Received: 29 January 2012; in revised form: 15 February 2012 / Accepted: 15 February 2012 / Published: 24 February 2012
(This article belongs to the Section Natural Products)
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Abstract: Baccharis dracunculifolia is a plant native from Brazil, commonly known as ‘Alecrim-do-campo’ and ‘Vassoura’ and used in alternative medicine for the treatment of inflammation, hepatic disorders and stomach ulcers. Previous studies reported that artepillin C (ArtC, 3-{4-hydroxy-3,5-di(3-methyl-2-butenyl)phenyl}-2(E)-propenoic acid), is the main compound of interest in the leaves. This study was undertaken to assess the mutagenic effect of the ethyl acetate extract of B. dracunculifolia leaves (Bd-EAE: 11.4–182.8 µg/plate) and ArtC (0.69–10.99 µg/plate) by the Ames test using Salmonella typhimurium strains TA98, TA97a, TA100 and TA102, and to compare the protective effects of Bd-EAE and ArtC against the mutagenicity of a variety of direct and indirect acting mutagens such as 4-nitro-O-phenylenediamine, sodium azide, mitomycin C, benzo[a]pyrene, aflatoxin B1, 2-aminoanthracene and 2-aminofluorene.The mutagenicity test showed that Bd-EAE and ArtC did not induce an increase in the number of revertant colonies indicating absence of mutagenic activity. ArtC showed a similar antimutagenic effect to that of Bd-EAE in some strains of S. typhimurium, demonstrating that the antimutagenic activity of Bd-EAE can be partially attributed to ArtC. The present results showed that the protective effect of whole plant extracts is due to the combined and synergistic effects of a complex mixture of phytochemicals, the total activity of which may result in health benefits.
Keywords: Baccharis dracunculifolia; artepillin C; Ames test; antimutagenicity; mutagenicity

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MDPI and ACS Style

Resende, F.A.; Munari, C.C.; de Azevedo Bentes Monteiro Neto, M.; Tavares, D.C.; Bastos, J.K.; da Silva Filho, A.A.; Varanda, E.A. Comparative Studies of the (Anti) Mutagenicity of Baccharis dracunculifolia and Artepillin C by the Bacterial Reverse Mutation Test. Molecules 2012, 17, 2335-2350.

AMA Style

Resende FA, Munari CC, de Azevedo Bentes Monteiro Neto M, Tavares DC, Bastos JK, da Silva Filho AA, Varanda EA. Comparative Studies of the (Anti) Mutagenicity of Baccharis dracunculifolia and Artepillin C by the Bacterial Reverse Mutation Test. Molecules. 2012; 17(3):2335-2350.

Chicago/Turabian Style

Resende, Flávia Aparecida; Munari, Carla Carolina; de Azevedo Bentes Monteiro Neto, Moacir; Tavares, Denise Crispim; Bastos, Jairo Kenupp; da Silva Filho, Ademar Alves; Varanda, Eliana Aparecida. 2012. "Comparative Studies of the (Anti) Mutagenicity of Baccharis dracunculifolia and Artepillin C by the Bacterial Reverse Mutation Test." Molecules 17, no. 3: 2335-2350.

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