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Molecules 2012, 17(2), 2240-2247; doi:10.3390/molecules17022240
Communication

2,3,5,4′- Tetrahydroxystilbene-2-O-β-D-glycoside Biosynthesis by Suspension Cells Cultures of Polygonum multiflorum Thunb and Production Enhancement by Methyl Jasmonate and Salicylic Acid

1,†
, 2,* , 1,†
, 1 and 1
1 School of Bioscience and bioengineering, South China University of Technology, Guangzhou 510006, China 2 General Hospital of Guangzhou Military Command, Guangzhou 510010, China These authors contributed equally to the work.
* Author to whom correspondence should be addressed.
Received: 9 January 2012 / Revised: 9 February 2012 / Accepted: 13 February 2012 / Published: 22 February 2012
(This article belongs to the Section Metabolites)
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Abstract

Friable calli of Polygonum multiflorum Thunb have been induced in MS medium supplemented with 6-benzylaminopurine (6-BA) and kinetin (KT). Suspension cultures were initiated from friable calli by inoculating calli in liquid MS medium in shake flasks in the dark and 25 °C on an orbital shaker at 100 rpm. The maximum dry weight (DW, 7.85 g/L) and 2,3,5,4′-tetrahydroxystilbene-2-O-β-D-glycoside (THSG, 56.39 mg/L) of suspension cells was obtained in MS medium after 16 days culture. Both methyl jasmonate (MeJA) and salicylic acid (SA) could increase THSG production. The most appropriate concentration of MeJA was 100 μmol/L in MS medium, in which concentration THSG content reached the maximum value of 147.79 mg/L, which represented a 162.36% increase compared to that of the control (56.33 mg/L). The most appropriate concentration of SA was 125 μmol/L in MS medium, at which concentration THSG content reached its maximum value of 116.43 mg/L, a 106.69% increase compared to that of the control (56.33 mg/L).
Keywords: Polygonum multiflorum Thunb; calli; suspension cells; THSG; MeJA; SA; elicitor Polygonum multiflorum Thunb; calli; suspension cells; THSG; MeJA; SA; elicitor
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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MDPI and ACS Style

Shao, L.; Zhao, S.-J.; Cui, T.-B.; Liu, Z.-Y.; Zhao, W. 2,3,5,4′- Tetrahydroxystilbene-2-O-β-D-glycoside Biosynthesis by Suspension Cells Cultures of Polygonum multiflorum Thunb and Production Enhancement by Methyl Jasmonate and Salicylic Acid. Molecules 2012, 17, 2240-2247.

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