Molecules 2012, 17(2), 1837-1851; doi:10.3390/molecules17021837
Article

Cytotoxic Phenylpropanoids and a New Triterpene, Turformosinic Acid, from Turpinia formosana Nakai

1,†, 2,†, 2, 2, 2, 2, 3, 4, 5,6 and 2,4,* email
Received: 26 January 2012; in revised form: 6 February 2012 / Accepted: 7 February 2012 / Published: 14 February 2012
(This article belongs to the Section Metabolites)
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Abstract: One new phenylpropanoid, turformosin A (1), and one new triterpene, turformosinic acid (2), together with 16 known compounds, were isolated from the stems of Turpinia formosana Nakai. All structures were elucidated on the basis of spectroscopic analysis, including 1D- and 2D-NMR techniques and MS analysis. Selected isolated compounds were evaluated for in vitro cytotoxicity against four human cancer cell lines and antioxidant scavenging effects on DPPH. (−)-(7′S,8′S)-threo-carolignan X (3) exhibited cytotoxicity against Hep2, WiDr, Daoy, and MCF-7 cell lines with ED50 values of 3.60, 4.45, 6.07, and 13.7 μg/mL, respectively. Turformosin A (1), (−)-(7′S,8′S)- threo-carolignan X (3), methoxyhydroquinone-4-β-d-glucopyranoside (5), and methoxy-hydroquinone-1-β-d-glucopyranoside (6), exhibited similar anti-oxidative activity. Hep2 cells treated with 10 μg/mL of 3 showed elevation of sub-G1 population (from 20% at 8 h to 60% at 48 h), and activation of caspase-9/caspase-3/PARP cascade. Compound 3 induced intrinsic apoptotic pathway in Hep2 cells with dose and time dependence (10 μg/mL for 8 h).
Keywords: Staphyleaceae; Turpinia formosana Nakai; turformosin A; turfomosinic acid; cytotoxicity
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MDPI and ACS Style

Huang, H.-C.; Chiou, C.-T.; Hsiao, P.-C.; Liaw, C.-C.; Zhang, L.-J.; Chang, C.-L.; Chen, I.-S.; Chen, W.-C.; Lee, K.-H.; Kuo, Y.-H. Cytotoxic Phenylpropanoids and a New Triterpene, Turformosinic Acid, from Turpinia formosana Nakai. Molecules 2012, 17, 1837-1851.

AMA Style

Huang H-C, Chiou C-T, Hsiao P-C, Liaw C-C, Zhang L-J, Chang C-L, Chen I-S, Chen W-C, Lee K-H, Kuo Y-H. Cytotoxic Phenylpropanoids and a New Triterpene, Turformosinic Acid, from Turpinia formosana Nakai. Molecules. 2012; 17(2):1837-1851.

Chicago/Turabian Style

Huang, Hui-Chi; Chiou, Chun-Tang; Hsiao, Ping-Chun; Liaw, Chia-Ching; Zhang, Li-Jie; Chang, Chao-Lin; Chen, Ih-Sheng; Chen, Wen-Chi; Lee, Kuo-Hsiung; Kuo, Yao-Haur. 2012. "Cytotoxic Phenylpropanoids and a New Triterpene, Turformosinic Acid, from Turpinia formosana Nakai." Molecules 17, no. 2: 1837-1851.

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