Next Article in Journal
Evaluation of the Antiproliferative Activity of the Leaves from Arctium lappa by a Bioassay-Guided Fractionation
Previous Article in Journal
Two New Iridoid Glycosides from the Root Barks of Sambucus williamsii Hance
Molecules 2012, 17(2), 1837-1851; doi:10.3390/molecules17021837
Article

Cytotoxic Phenylpropanoids and a New Triterpene, Turformosinic Acid, from Turpinia formosana Nakai

1,†, 2,†, 2, 2, 2, 2, 3, 4, 5,6 and 2,4,*
1 School of Chinese Pharmaceutical Sciences and Chinese Medicine Resources, China Medical University, Taichung 404, Taiwan 2 Division of Herbal Drug and Natural Products, National Research Institute of Chinese Medicine, Taipei 112, Taiwan 3 College of Pharmacy, Kaohsiung Medical University, Kaohsiung 807, Taiwan 4 Graduate Institute of Integrated Medicine, College of Chinese Medicine, China Medical University, Taichung 404, Taiwan 5 Natural Products Research Laboratories, UNC Eshelman School of Pharmacy, University of North Carolina, Chapel Hill, NC 27599, USA 6 Chinese Medicine Research and Development Center, China Medical University and Hospital, Taichung 404, Taiwan These authors contribute equally to this work.
* Author to whom correspondence should be addressed.
Received: 26 January 2012 / Revised: 6 February 2012 / Accepted: 7 February 2012 / Published: 14 February 2012
(This article belongs to the Section Metabolites)
Download PDF [433 KB, uploaded 18 June 2014]

Abstract

One new phenylpropanoid, turformosin A (1), and one new triterpene, turformosinic acid (2), together with 16 known compounds, were isolated from the stems of Turpinia formosana Nakai. All structures were elucidated on the basis of spectroscopic analysis, including 1D- and 2D-NMR techniques and MS analysis. Selected isolated compounds were evaluated for in vitro cytotoxicity against four human cancer cell lines and antioxidant scavenging effects on DPPH. (−)-(7′S,8′S)-threo-carolignan X (3) exhibited cytotoxicity against Hep2, WiDr, Daoy, and MCF-7 cell lines with ED50 values of 3.60, 4.45, 6.07, and 13.7 μg/mL, respectively. Turformosin A (1), (−)-(7′S,8′S)- threo-carolignan X (3), methoxyhydroquinone-4-β-d-glucopyranoside (5), and methoxy-hydroquinone-1-β-d-glucopyranoside (6), exhibited similar anti-oxidative activity. Hep2 cells treated with 10 μg/mL of 3 showed elevation of sub-G1 population (from 20% at 8 h to 60% at 48 h), and activation of caspase-9/caspase-3/PARP cascade. Compound 3 induced intrinsic apoptotic pathway in Hep2 cells with dose and time dependence (10 μg/mL for 8 h).
Keywords: Staphyleaceae; Turpinia formosana Nakai; turformosin A; turfomosinic acid; cytotoxicity Staphyleaceae; Turpinia formosana Nakai; turformosin A; turfomosinic acid; cytotoxicity
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Share & Cite This Article

Export to BibTeX |
EndNote


MDPI and ACS Style

Huang, H.-C.; Chiou, C.-T.; Hsiao, P.-C.; Liaw, C.-C.; Zhang, L.-J.; Chang, C.-L.; Chen, I.-S.; Chen, W.-C.; Lee, K.-H.; Kuo, Y.-H. Cytotoxic Phenylpropanoids and a New Triterpene, Turformosinic Acid, from Turpinia formosana Nakai. Molecules 2012, 17, 1837-1851.

View more citation formats

Related Articles

Article Metrics

Comments

Citing Articles

[Return to top]
Molecules EISSN 1420-3049 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert