Next Article in Journal
One-Pot Ugi/Aza-Michael Synthesis of Highly Substituted 2,5-Diketopiperazines with Anti-Proliferative Properties
Previous Article in Journal
Synthesis and Pharmacological Evaluation of Novel Phenyl Sulfonamide Derivatives Designed as Modulators of Pulmonary Inflammatory Response
Molecules 2012, 17(12), 14673-14684; doi:10.3390/molecules171214673
Article

Wu-Chia-Pi Solution Attenuates Carbon Tetrachloride-Induced Hepatic Injury through the Antioxidative Abilities of Its Components Acteoside and Quercetin

1,2
, 3,4
, 3
, 3
, 3
 and 3,5,*
1 Division of Urology, Department of Surgery, Chi Mei Medical Center, No.21, Taikang, Liuying Dist., Tainan 73657, Taiwan 2 Graduate Institute of Clinical Medical Research, Taipei Medical University, 250 Wu-Hsing Street, Taipei 11031, Taiwan 3 School of Pharmacy, College of Pharmacy, Taipei Medical University, 250 Wu-Hsing Street, Taipei 11031, Taiwan 4 Section of Biologics & Advanced Therapeutic Product Analysis, Division of Research and Analysis, Food and Drug Administration, No.161-2, Kunyang St, Nangang District, Taipei 11561, Taiwan 5 Orthopedics Research Center, Taipei Medical University Hospital, Taipei 11031, Taiwan
* Author to whom correspondence should be addressed.
Received: 8 October 2012 / Revised: 6 December 2012 / Accepted: 7 December 2012 / Published: 11 December 2012
(This article belongs to the Section Metabolites)
Download PDF [296 KB, uploaded 18 June 2014]

Abstract

Wu-Chia-Pi medicated wine, composed nine Chinese medicines soaked in 35% alcohol, is widely used in Asia for its health-promoting functions. However, long-term consumption of alcohol could result in liver dysfunction. In this study, Wu-Chia-Pi solution (WCPS) and extract (WCPE) were prepared by modification of the principals given by the Committee on Chinese Medicine and Pharmacy in Taiwan. The aim of this study was to explore the protective effect of WCPS against carbon tetrachloride (CCl4)-induced liver injury and to clarify its active component(s). Antioxidative effects of the test samples were evaluated via MDA inhibition, catalase activity and DPPH-scavenging assays. HPLC was used to analysis the active components. Results showed that WCPS (1 and 5 mL/kg) significantly prevented CCl4-induced liver injury without chronic liver toxicity. Referring to the antioxidative activities, WCPE displayed significant MDA inhibitory and DPPH-scavenging activities with IC50 values of 0.91 ± 0.03 and 0.60 ± 0.04 mg/mL, respectively. Catalase activity was also enhanced by treatment of WCPE, acteoside and quercetin. Therefore, we suggest that acteoside and quercetin are the major contributors to the antioxidative and hepatoprotective activities of WCPS, and a possible mechanism could be mediated through reduction of oxidative stress.
Keywords: Wu-Chia-Pi solution; antioxidative activities; hepatoprotection; carbon tetrachloride; acteoside; quercetin Wu-Chia-Pi solution; antioxidative activities; hepatoprotection; carbon tetrachloride; acteoside; quercetin
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Share & Cite This Article

Export to BibTeX |
EndNote


MDPI and ACS Style

Huan, S.K.-H.; Wang, K.-T.; Lee, C.-J.; Sung, C.-H.; Chien, T.-Y.; Wang, C.-C. Wu-Chia-Pi Solution Attenuates Carbon Tetrachloride-Induced Hepatic Injury through the Antioxidative Abilities of Its Components Acteoside and Quercetin. Molecules 2012, 17, 14673-14684.

View more citation formats

Related Articles

Article Metrics

Comments

Citing Articles

[Return to top]
Molecules EISSN 1420-3049 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert