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An Electrostatically Crosslinked Chitosan Hydrogel as a Drug Carrier
Hankuk Academy of Foreign Studies, Yongin 449-854, Korea
Department of Molecular Science and Technology, Ajou University, Suwon 443-749, Korea
* Author to whom correspondence should be addressed.
Received: 17 October 2012; in revised form: 9 November 2012 / Accepted: 12 November 2012 / Published: 22 November 2012
Abstract: Considerable efforts have been devoted to control and maintain the sustained release of proteins. In this experiment, we used bovine serum albumin-fluorescein isothiocyanate (BSA-FITC) as a model protein to explore the potential utility of a chitosan and glycerol phosphate disodium salt (GP) hydrogel as a protein drug depot. The mixing of chitosan and GP solutions (0, 10, 20 and 30 wt%) formed a liquid at room temperature. At 37 °C, however, the chitosan/GP solutions formed hydrogels through an electrostatic crosslinking process. This electrostatic interaction between the chitosan, cationic amine group, and GP, anionic phosphate group, was confirmed by the changes of zeta potentials and particle sizes of this solution. The electrostatic interaction depended both on the GP ratios in chitosan and the incubation time of chitosan/GP solutions. Furthermore, BSA-FITC-loaded chitosan/GP hydrogels were examined for their ability as potential depots for the BSA drugs. Hence, when observed, the BSA-FITC-loaded chitosan/GP hydrogels showed an in vitro sustained release profile of BSA up to 14 days. Collectively, our results show that the chitosan/GP hydrogels described here, can serve as depots for BSA drugs.
Keywords: chitosan; glycerol phosphate disodium salt; hydrogel; protein; depot
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MDPI and ACS Style
Kim, G.O.; Kim, N.; Kim, D.Y.; Kwon, J.S.; Min, B.-H. An Electrostatically Crosslinked Chitosan Hydrogel as a Drug Carrier. Molecules 2012, 17, 13704-13711.
Kim GO, Kim N, Kim DY, Kwon JS, Min B-H. An Electrostatically Crosslinked Chitosan Hydrogel as a Drug Carrier. Molecules. 2012; 17(12):13704-13711.
Kim, Ga O.; Kim, Nawoo; Kim, Da Y.; Kwon, Jin S.; Min, Byoung-Hyun. 2012. "An Electrostatically Crosslinked Chitosan Hydrogel as a Drug Carrier." Molecules 17, no. 12: 13704-13711.