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10-Acetylirciformonin B, A Sponge Furanoterpenoid, Induces DNA Damage and Apoptosis in Leukemia Cells
Graduate Institute of Marine Biotechnology, National Dong Hwa University, Pingtung 944, Taiwan
National Museum of Marine Biology & Aquarium, Pingtung 944, Taiwan
Institute of Marine Biodiversity and Evolution, National Dong Hwa University, Pingtung 944, Taiwan
Department of Nutrition, Lee’s Endocrinology Clinics, Pintung 900, Taiwan
Graduate Institute of Natural Products, College of Pharmacy, Kaohsiung Medical University, Kaohsiung 807, Taiwan
Department of Pharmacognosy and Natural Products Chemistry, Faculty of Pharmacy, Ain-Shams University, Organization of African Unity Street, Abassia, Cairo 11566, Egypt
Eastern Marine Biology Research Center, Fisheries Research Institute, Taitung 961, Taiwan
* Author to whom correspondence should be addressed.
Received: 1 August 2012; in revised form: 16 August 2012 / Accepted: 14 September 2012 / Published: 9 October 2012
Abstract: 10-Acetylirciformonin B, a furanoterpenoid derived from irciformonin B found in a marine sponge, has been reported to possess potent cytotoxic activity against several cancer cell lines. However, the mechanism of its apoptotic activity against human leukemia cells has never been reported. The purpose of this study was to investigate the cytotoxic effects of 10-acetylirciformonin B and its possible mechanism of action against leukemia HL 60 cells. We found that 10-acetylirciformonin B decreased cell viability through the inhibition of cell growth as well as the induction of DNA damage and apoptosis in a dose-dependent manner. The induction of DNA damage was mediated by the increase of p-CHK2 and γ-H2A.X, which was suggested from the increase of tail movement in the neutral Comet assay. Induction of apoptosis was mediated with the increase in caspases 8, 9 and 3 activation as well as PARP cleavage. In summary, our resultsindicate that 10-acetylirciformonin B treatment causes apoptosis in leukaemia cells; probably through a caspase-dependent regulatory pathway.
Keywords: 10-acetylirciformonin B; apoptosis; DNA damage; sponge
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Su, J.-H.; Chang, W.-B.; Chen, H.-M.; El-Shazly, M.; Du, Y.-C.; Kung, T.-H.; Chen, Y.-C.; Sung, P.-J.; Ho, Y.-S.; Kuo, F.-W.; Lu, M.-C. 10-Acetylirciformonin B, A Sponge Furanoterpenoid, Induces DNA Damage and Apoptosis in Leukemia Cells. Molecules 2012, 17, 11839-11848.
Su J-H, Chang W-B, Chen H-M, El-Shazly M, Du Y-C, Kung T-H, Chen Y-C, Sung P-J, Ho Y-S, Kuo F-W, Lu M-C. 10-Acetylirciformonin B, A Sponge Furanoterpenoid, Induces DNA Damage and Apoptosis in Leukemia Cells. Molecules. 2012; 17(10):11839-11848.
Su, Jui-Hsin; Chang, Wen-Been; Chen, Huei-Mei; El-Shazly, Mohamed; Du, Ying-Chi; Kung, Ting-Hsuan; Chen, Yu-Cheng; Sung, Ping-Jyun; Ho, Yuan-Shing; Kuo, Fu-Wen; Lu, Mei-Chin. 2012. "10-Acetylirciformonin B, A Sponge Furanoterpenoid, Induces DNA Damage and Apoptosis in Leukemia Cells." Molecules 17, no. 10: 11839-11848.