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Molecules 2012, 17(10), 11839-11848; doi:10.3390/molecules171011839
Article

10-Acetylirciformonin B, A Sponge Furanoterpenoid, Induces DNA Damage and Apoptosis in Leukemia Cells

1,2, 2,3, 4, 5,6, 5, 1, 1, 1,2, 7, 2 and 1,2,*
Received: 1 August 2012; in revised form: 16 August 2012 / Accepted: 14 September 2012 / Published: 9 October 2012
(This article belongs to the Section Metabolites)
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Abstract: 10-Acetylirciformonin B, a furanoterpenoid derived from irciformonin B found in a marine sponge, has been reported to possess potent cytotoxic activity against several cancer cell lines. However, the mechanism of its apoptotic activity against human leukemia cells has never been reported. The purpose of this study was to investigate the cytotoxic effects of 10-acetylirciformonin B and its possible mechanism of action against leukemia HL 60 cells. We found that 10-acetylirciformonin B decreased cell viability through the inhibition of cell growth as well as the induction of DNA damage and apoptosis in a dose-dependent manner. The induction of DNA damage was mediated by the increase of p-CHK2 and γ-H2A.X, which was suggested from the increase of tail movement in the neutral Comet assay. Induction of apoptosis was mediated with the increase in caspases 8, 9 and 3 activation as well as PARP cleavage. In summary, our resultsindicate that 10-acetylirciformonin B treatment causes apoptosis in leukaemia cells; probably through a caspase-dependent regulatory pathway.
Keywords: 10-acetylirciformonin B; apoptosis; DNA damage; sponge 10-acetylirciformonin B; apoptosis; DNA damage; sponge
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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MDPI and ACS Style

Su, J.-H.; Chang, W.-B.; Chen, H.-M.; El-Shazly, M.; Du, Y.-C.; Kung, T.-H.; Chen, Y.-C.; Sung, P.-J.; Ho, Y.-S.; Kuo, F.-W.; Lu, M.-C. 10-Acetylirciformonin B, A Sponge Furanoterpenoid, Induces DNA Damage and Apoptosis in Leukemia Cells. Molecules 2012, 17, 11839-11848.

AMA Style

Su J-H, Chang W-B, Chen H-M, El-Shazly M, Du Y-C, Kung T-H, Chen Y-C, Sung P-J, Ho Y-S, Kuo F-W, Lu M-C. 10-Acetylirciformonin B, A Sponge Furanoterpenoid, Induces DNA Damage and Apoptosis in Leukemia Cells. Molecules. 2012; 17(10):11839-11848.

Chicago/Turabian Style

Su, Jui-Hsin; Chang, Wen-Been; Chen, Huei-Mei; El-Shazly, Mohamed; Du, Ying-Chi; Kung, Ting-Hsuan; Chen, Yu-Cheng; Sung, Ping-Jyun; Ho, Yuan-Shing; Kuo, Fu-Wen; Lu, Mei-Chin. 2012. "10-Acetylirciformonin B, A Sponge Furanoterpenoid, Induces DNA Damage and Apoptosis in Leukemia Cells." Molecules 17, no. 10: 11839-11848.



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