Abstract: Leptadenia pyrotechnica Forssk is a traditional medicinal herb used for treatment of inflammatory diseases and cancer. In this research, the aqueous ethanolic crude extract of Leptadenia pyrotechnica aerial parts, along with its ethyl acetate, n-butanol and water partitioning fractions were evaluated for their antioxidant capacity, polyphenolic content, anti-inflammatory and anti-cancer properties. The total antioxidant capacity was estimated by the FRAP, DPPH, ABTS and β-carotene bleaching assays.The ethyl acetate fraction exhibited the highest polyphenolic content (252.27 mg gallic acid/g) and the best antioxidant activity (1.2, 0.57, 0.45 mmol ascorbic acid equivalent/g in the FRAP, ABTS and DPPH assays, respectively). Furthermore, the same extract showed appreciable anti-inflammatory via lipoxygenase (LOX) inhibitory activity (IC50 = 1.41 µg/mL). Moreover, the ethyl acetate fraction also showed the strongest cytotoxic effect (IC50 = 43.16 µg/mL) against MCF-7 human breast cancer cell line. These results suggest that this plant may be considered an interesting source of compounds with antioxidant, anti-inflammatory and anti-cancer properties for therapeutic, nutraceutical and functional food applications.
This is an open access article distributed under the
Creative Commons Attribution License which permits unrestricted use, distribution,
and reproduction in any medium, provided the original work is properly cited.
Export to BibTeX
MDPI and ACS Style
Khasawneh, M.A.; Elwy, H.M.; Hamza, A.A.; Fawzi, N.M.; Hassan, A.H. Antioxidant, Anti-Lipoxygenase and Cytotoxic Activity of Leptadenia pyrotechnica (Forssk.) Decne Polyphenolic Constituents. Molecules 2011, 16, 7510-7521.
Khasawneh MA, Elwy HM, Hamza AA, Fawzi NM, Hassan AH. Antioxidant, Anti-Lipoxygenase and Cytotoxic Activity of Leptadenia pyrotechnica (Forssk.) Decne Polyphenolic Constituents. Molecules. 2011; 16(9):7510-7521.
Khasawneh, Mohammad A.; Elwy, Hanan M.; Hamza, Alaaeldin A.; Fawzi, Nael M.; Hassan, Ahmed H. 2011. "Antioxidant, Anti-Lipoxygenase and Cytotoxic Activity of Leptadenia pyrotechnica (Forssk.) Decne Polyphenolic Constituents." Molecules 16, no. 9: 7510-7521.