Abstract: Leptadenia pyrotechnica Forssk is a traditional medicinal herb used for treatment of inflammatory diseases and cancer. In this research, the aqueous ethanolic crude extract of Leptadenia pyrotechnica aerial parts, along with its ethyl acetate, n-butanol and water partitioning fractions were evaluated for their antioxidant capacity, polyphenolic content, anti-inflammatory and anti-cancer properties. The total antioxidant capacity was estimated by the FRAP, DPPH, ABTS and β-carotene bleaching assays.The ethyl acetate fraction exhibited the highest polyphenolic content (252.27 mg gallic acid/g) and the best antioxidant activity (1.2, 0.57, 0.45 mmol ascorbic acid equivalent/g in the FRAP, ABTS and DPPH assays, respectively). Furthermore, the same extract showed appreciable anti-inflammatory via lipoxygenase (LOX) inhibitory activity (IC50 = 1.41 µg/mL). Moreover, the ethyl acetate fraction also showed the strongest cytotoxic effect (IC50 = 43.16 µg/mL) against MCF-7 human breast cancer cell line. These results suggest that this plant may be considered an interesting source of compounds with antioxidant, anti-inflammatory and anti-cancer properties for therapeutic, nutraceutical and functional food applications.
Keywords: Leptadenia pyrotechnica; total phenol; antioxidant; anti-inflammatory; cytotoxicity
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Khasawneh, M.A.; Elwy, H.M.; Hamza, A.A.; Fawzi, N.M.; Hassan, A.H. Antioxidant, Anti-Lipoxygenase and Cytotoxic Activity of Leptadenia pyrotechnica (Forssk.) Decne Polyphenolic Constituents. Molecules 2011, 16, 7510-7521.
Khasawneh MA, Elwy HM, Hamza AA, Fawzi NM, Hassan AH. Antioxidant, Anti-Lipoxygenase and Cytotoxic Activity of Leptadenia pyrotechnica (Forssk.) Decne Polyphenolic Constituents. Molecules. 2011; 16(9):7510-7521.
Khasawneh, Mohammad A.; Elwy, Hanan M.; Hamza, Alaaeldin A.; Fawzi, Nael M.; Hassan, Ahmed H. 2011. "Antioxidant, Anti-Lipoxygenase and Cytotoxic Activity of Leptadenia pyrotechnica (Forssk.) Decne Polyphenolic Constituents." Molecules 16, no. 9: 7510-7521.