Molecules 2011, 16(6), 5054-5061; doi:10.3390/molecules16065054
Article

Cholesterol-Lowering Activity of the Major Polyphenols in Grape Seed

1 The Medical Food Research and Development Center, Department of Transfusion Medicine, Faculty of Allied Health Sciences, Chulalongkorn University, Bangkok, Thailand 2 Program in Nutrition and Dietetics, Faculty of Allied Health Sciences, Chulalongkorn University, Bangkok, Thailand 3 The Research Group of Herbal Medicine for Prevention and Therapeutic of Metabolic Diseases, Chulalongkorn University, Bangkok, Thailand
* Author to whom correspondence should be addressed.
Received: 8 April 2011; in revised form: 13 June 2011 / Accepted: 16 June 2011 / Published: 17 June 2011
(This article belongs to the Section Natural Products)
PDF Full-text Download PDF Full-Text [262 KB, uploaded 17 June 2011 14:04 CEST]
Abstract: The major polyphenols in grape seed have been shown to have beneficial health effects in the prevention of dyslipidemia and cardiovascular diseases. In this present study, we investigated the cholesterol-lowering activity of three major polyphenolic compounds found in grape seed. The results showed that gallic acid, catechin, and epicatechin significantly inhibited pancreatic cholesterol esterase in a concentration-dependent manner. Moreover, they bound to taurocholic acid, taurodeoxycholic acid, and glycodeoxycholic acid at levels ranging from 38.6% to 28.2%. At the concentration of 0.2 mg/mL, gallic acid, catechin, and epicatechin reduced the formation of cholesterol micelles 27.26 ± 2.17%, 11.88 ± 0.75%, and 19.49 ± 3.71%, respectively. These findings clearly demonstrate that three major polyphenolic compounds present in a particular grape seed have cholesterol-lowering activity by inhibiting pancreatic cholesterol esterase, binding of bile acids, and reducing solubility of cholesterol in micelles which may result in delayed cholesterol absorption.
Keywords: polyphenols; grape seed; mechanism pancreatic cholesterol esterase; cholesterol micelles; bile acid

Article Statistics

Load and display the download statistics.

Citations to this Article

Cite This Article

MDPI and ACS Style

Ngamukote, S.; Mäkynen, K.; Thilawech, T.; Adisakwattana, S. Cholesterol-Lowering Activity of the Major Polyphenols in Grape Seed. Molecules 2011, 16, 5054-5061.

AMA Style

Ngamukote S, Mäkynen K, Thilawech T, Adisakwattana S. Cholesterol-Lowering Activity of the Major Polyphenols in Grape Seed. Molecules. 2011; 16(6):5054-5061.

Chicago/Turabian Style

Ngamukote, Sathaporn; Mäkynen, Kittana; Thilawech, Thavaree; Adisakwattana, Sirichai. 2011. "Cholesterol-Lowering Activity of the Major Polyphenols in Grape Seed." Molecules 16, no. 6: 5054-5061.

Molecules EISSN 1420-3049 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert