Molecules 2011, 16(6), 5054-5061; doi:10.3390/molecules16065054
Article

Cholesterol-Lowering Activity of the Major Polyphenols in Grape Seed

Received: 8 April 2011; in revised form: 13 June 2011 / Accepted: 16 June 2011 / Published: 17 June 2011
(This article belongs to the Section Natural Products)
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Abstract: The major polyphenols in grape seed have been shown to have beneficial health effects in the prevention of dyslipidemia and cardiovascular diseases. In this present study, we investigated the cholesterol-lowering activity of three major polyphenolic compounds found in grape seed. The results showed that gallic acid, catechin, and epicatechin significantly inhibited pancreatic cholesterol esterase in a concentration-dependent manner. Moreover, they bound to taurocholic acid, taurodeoxycholic acid, and glycodeoxycholic acid at levels ranging from 38.6% to 28.2%. At the concentration of 0.2 mg/mL, gallic acid, catechin, and epicatechin reduced the formation of cholesterol micelles 27.26 ± 2.17%, 11.88 ± 0.75%, and 19.49 ± 3.71%, respectively. These findings clearly demonstrate that three major polyphenolic compounds present in a particular grape seed have cholesterol-lowering activity by inhibiting pancreatic cholesterol esterase, binding of bile acids, and reducing solubility of cholesterol in micelles which may result in delayed cholesterol absorption.
Keywords: polyphenols; grape seed; mechanism pancreatic cholesterol esterase; cholesterol micelles; bile acid
PDF Full-text Download PDF Full-Text [262 KB, uploaded 18 June 2014 19:38 CEST]

Export to BibTeX |
EndNote


MDPI and ACS Style

Ngamukote, S.; Mäkynen, K.; Thilawech, T.; Adisakwattana, S. Cholesterol-Lowering Activity of the Major Polyphenols in Grape Seed. Molecules 2011, 16, 5054-5061.

AMA Style

Ngamukote S, Mäkynen K, Thilawech T, Adisakwattana S. Cholesterol-Lowering Activity of the Major Polyphenols in Grape Seed. Molecules. 2011; 16(6):5054-5061.

Chicago/Turabian Style

Ngamukote, Sathaporn; Mäkynen, Kittana; Thilawech, Thavaree; Adisakwattana, Sirichai. 2011. "Cholesterol-Lowering Activity of the Major Polyphenols in Grape Seed." Molecules 16, no. 6: 5054-5061.

Molecules EISSN 1420-3049 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert