Molecules 2011, 16(5), 4254-4263; doi:10.3390/molecules16054254
Article

Cytotoxicity and Pro-Apoptotic Activity of 2,2´-Bis[4,5-bis(4-hydroxybenzyl)-2-(4-hydroxyphenyl)cyclopent-4-en-1,3-dione], a Phenolic Cyclopentenedione Isolated from the Cyanobacterium Strain Nostoc sp. str. Lukešová 27/97

Jan Vacek 1 , Jan Hrbáč 2 , Jiří Kopecký 3  and Jitka Vostálová 1,* email
1 Department of Medical Chemistry and Biochemistry, Faculty of Medicine and Dentistry, Palacký University, Hněvotínská 3, 775 15 Olomouc, Czech Republic 2 Department Physical Chemistry, Faculty of Science, Palacký University, tř. 17.listopadu 12, 771 46 Olomouc, Czech Republic 3 Department of Autotrophic Microorganisms, Institute of Microbiology, Academy of Sciences of the Czech Republic, Opatovický mlýn, 379 81 Třeboň, Czech Republic
* Author to whom correspondence should be addressed.
Received: 14 March 2011; in revised form: 13 May 2011 / Accepted: 18 May 2011 / Published: 23 May 2011
(This article belongs to the Special Issue Bioactive Compounds)
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Abstract: The cytotoxicity of the polyphenol 2,2´-bis[4,5-bis(4-hydroxybenzyl)-2-(4-hydroxyphenyl)cyclopent-4-en-1,3-dione], nostotrebin 6 (NOS-6), was tested under in vitro conditions using mouse fibroblasts (BALB/c cells). Identification of NOS-6 and its uptake into fibroblasts was examined by multi-stage mass spectrometry analysis with the following fragmentation pattern: MS (m/z) [M+H]+ 799.1 → MS2 399.1 → MS3 305.1 → MS4 277.1. Using several cell viability assays, the IC50 of NOS-6 after 24 h incubation was found to be 8.48 ± 0.16/12.15 ± 1.96 µM (neutral red/MTT assay) which was higher than that of doxorubicin. It was found that NOS-6 is capable of inducing both types of cell death, apoptosis and necrosis in a dose-dependent manner. The biological activities of the cyclopentenediones and preliminary data on NOS-6 cytotoxicity are discussed.
Keywords: polyphenol; cyclopentenediones; cytotoxicity; apoptosis; necrosis

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MDPI and ACS Style

Vacek, J.; Hrbáč, J.; Kopecký, J.; Vostálová, J. Cytotoxicity and Pro-Apoptotic Activity of 2,2´-Bis[4,5-bis(4-hydroxybenzyl)-2-(4-hydroxyphenyl)cyclopent-4-en-1,3-dione], a Phenolic Cyclopentenedione Isolated from the Cyanobacterium Strain Nostoc sp. str. Lukešová 27/97. Molecules 2011, 16, 4254-4263.

AMA Style

Vacek J, Hrbáč J, Kopecký J, Vostálová J. Cytotoxicity and Pro-Apoptotic Activity of 2,2´-Bis[4,5-bis(4-hydroxybenzyl)-2-(4-hydroxyphenyl)cyclopent-4-en-1,3-dione], a Phenolic Cyclopentenedione Isolated from the Cyanobacterium Strain Nostoc sp. str. Lukešová 27/97. Molecules. 2011; 16(5):4254-4263.

Chicago/Turabian Style

Vacek, Jan; Hrbáč, Jan; Kopecký, Jiří; Vostálová, Jitka. 2011. "Cytotoxicity and Pro-Apoptotic Activity of 2,2´-Bis[4,5-bis(4-hydroxybenzyl)-2-(4-hydroxyphenyl)cyclopent-4-en-1,3-dione], a Phenolic Cyclopentenedione Isolated from the Cyanobacterium Strain Nostoc sp. str. Lukešová 27/97." Molecules 16, no. 5: 4254-4263.

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