Detection of Cytotoxic Activity of Lectin on Human Colon Adenocarcinoma (Sw480) and Epithelial Cervical Carcinoma (C33-A)
Abstract
:1. Introduction
2. Results and Discussion
2.1. Tepary bean lectin purification
Lectin | Lectin Concentration (mg/mL) | Human erythrocytes | |
---|---|---|---|
Type A | Type O | ||
Tepary | 3.5 | 292.6 ± 32.9 | 36.6 ± 4.3 |
2.2. Effect of the TBL on the viability of human malignant cells
2.3. Cytotoxic effect of TBL in human malignant cells
Treatment | DNA (% incorporation 3[H] thymidine) | |
---|---|---|
SW480 | C33-A | |
Control | 100 ± 10.56 | 100 ± 11.06 |
10 µg/mL | 71.3 ± 5.93 | 42.6 ± 11.35 |
25 µg/mL | 66.7 ± 6.19 | 23.6 ± 12.08 |
50 µg/mL | 59.7 ± 5.43 | 21.2 ± 13.47 |
100 µg/mL | 33.4 ± 18.77 | 43.1 ± 4.96 |
2.4. Colony formation
2.5. Discussion
3. Experimental
3.1. Extraction and purification of lectins
3.2. Quantification of protein
3.3. Hemagglutination assays
3.4. Polyacrilamide gel electrophoresis (PAGE)
3.5. Cell culture
3.6. Cell viability assay
3.7. Cytotoxicity assay
3.8. Colony formation assay
4. Conclusions
References
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Valadez-Vega, C.; Alvarez-Manilla, G.; Riverón-Negrete, L.; García-Carrancá, A.; Morales-González, J.A.; Zuñiga-Pérez, C.; Madrigal-Santillán, E.; Esquivel-Soto, J.; Esquivel-Chirino, C.; Villagómez-Ibarra, R.; et al. Detection of Cytotoxic Activity of Lectin on Human Colon Adenocarcinoma (Sw480) and Epithelial Cervical Carcinoma (C33-A). Molecules 2011, 16, 2107-2118. https://doi.org/10.3390/molecules16032107
Valadez-Vega C, Alvarez-Manilla G, Riverón-Negrete L, García-Carrancá A, Morales-González JA, Zuñiga-Pérez C, Madrigal-Santillán E, Esquivel-Soto J, Esquivel-Chirino C, Villagómez-Ibarra R, et al. Detection of Cytotoxic Activity of Lectin on Human Colon Adenocarcinoma (Sw480) and Epithelial Cervical Carcinoma (C33-A). Molecules. 2011; 16(3):2107-2118. https://doi.org/10.3390/molecules16032107
Chicago/Turabian StyleValadez-Vega, Carmen, Gerardo Alvarez-Manilla, Leticia Riverón-Negrete, Alejandro García-Carrancá, José A. Morales-González, Clara Zuñiga-Pérez, Eduardo Madrigal-Santillán, Jaime Esquivel-Soto, Cesar Esquivel-Chirino, Roberto Villagómez-Ibarra, and et al. 2011. "Detection of Cytotoxic Activity of Lectin on Human Colon Adenocarcinoma (Sw480) and Epithelial Cervical Carcinoma (C33-A)" Molecules 16, no. 3: 2107-2118. https://doi.org/10.3390/molecules16032107